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Cytolytic efficiency of rabbit-anti-mouse antilymphocytic globulin and its augmentation by antiglobulin
The cytotoxic titre −1 of rabbit-anti-mouse thymocyte IgG (ALG), determined by a modified test in which the cells were first incubated with ALG, then washed and re-incubated with guinea-pig C, ranged from 3110 to 5470. The cytolytic efficiency (i.e. the reciprocal of the mean number of IgG molecules...
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| Published in: | Clinical and experimental immunology 1971-12, Vol.9 (6), p.839-851 |
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| Main Authors: | , |
| Format: | Article |
| Language: | English |
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| container_end_page | 851 |
| container_issue | 6 |
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| container_title | Clinical and experimental immunology |
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| creator | Woodruff, M F Inchley, M P |
| description | The cytotoxic titre
−1
of rabbit-anti-mouse thymocyte IgG (ALG), determined by a modified test in which the cells were first incubated with ALG, then washed and re-incubated with guinea-pig C, ranged from 3110 to 5470. The cytolytic efficiency (i.e. the reciprocal of the mean number of IgG molecules attached per cell under conditions in which 50% lysis occurs in the presence of C), measured with
131
I-ALG, was about 0·002%. When γ
2
IgG from guinea-pigs immunized with rabbit IgG was added prior to the second incubation the cytotoxic titre was augmented up to ten-fold and the cytotoxic efficiency up to twenty-fold. The same γ
2
preparation resulted in up to 300-fold augmentation in a haemolytic system. In the thymocyte-ALG system, unlike the haemolytic system, augmentation was maximal at reduced concentrations of γ
2
. The mechanism of augmentation by γ
2
has been investigated using
131
I-ALG and
125
I- γ
2
IgG. |
| format | article |
| fullrecord | <record><control><sourceid>pubmed</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1713139</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>5316638</sourcerecordid><originalsourceid>FETCH-LOGICAL-p261t-8f9c419451f609db6223a095ae1008f851e84a8d2a4faf26f3ba5f75d107f20b3</originalsourceid><addsrcrecordid>eNpVkN1KxDAQhYMoa119BCEvEMhPkzY3ghR_Fha80esyaZNupG1Kmwp9e-u6il7NDOecj8OcoYQJJQnnqT5HCaVUE81oeomupul9PZVSfIM2UjClRJ6gplhiaJfoK2yd85W3fbXg4PAIxvhIoI-edGGeLP5a26UbDqE6-ps2mLn1_SrU2McJw9x0to8QfeixWY6BH9M1unDQTvbmNLfo7fHhtXgm-5enXXG_JwNXLJLc6SplOpXMKaprozgXQLUEyyjNXS6ZzVPIaw6pA8eVEwaky2TNaOY4NWKL7r65w2w6W1drnxHachh9B-NSBvDlf6X3h7IJHyXLmGBCr4Dbv4Df5Olj4hNs92yN</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Cytolytic efficiency of rabbit-anti-mouse antilymphocytic globulin and its augmentation by antiglobulin</title><source>Open Access: PubMed Central</source><creator>Woodruff, M F ; Inchley, M P</creator><creatorcontrib>Woodruff, M F ; Inchley, M P</creatorcontrib><description>The cytotoxic titre
−1
of rabbit-anti-mouse thymocyte IgG (ALG), determined by a modified test in which the cells were first incubated with ALG, then washed and re-incubated with guinea-pig C, ranged from 3110 to 5470. The cytolytic efficiency (i.e. the reciprocal of the mean number of IgG molecules attached per cell under conditions in which 50% lysis occurs in the presence of C), measured with
131
I-ALG, was about 0·002%. When γ
2
IgG from guinea-pigs immunized with rabbit IgG was added prior to the second incubation the cytotoxic titre was augmented up to ten-fold and the cytotoxic efficiency up to twenty-fold. The same γ
2
preparation resulted in up to 300-fold augmentation in a haemolytic system. In the thymocyte-ALG system, unlike the haemolytic system, augmentation was maximal at reduced concentrations of γ
2
. The mechanism of augmentation by γ
2
has been investigated using
131
I-ALG and
125
I- γ
2
IgG.</description><identifier>ISSN: 0009-9104</identifier><identifier>EISSN: 1365-2249</identifier><identifier>PMID: 5316638</identifier><language>eng</language><publisher>England</publisher><subject>Animals ; Antibodies, Anti-Idiotypic ; Antilymphocyte Serum ; Chromatography, DEAE-Cellulose ; Complement System Proteins ; Cytotoxicity Tests, Immunologic ; Goats ; Guinea Pigs ; Immunization ; Immunoelectrophoresis ; Immunoglobulin G ; Iodine Isotopes ; Mice ; Rabbits ; Thymus Gland - cytology ; Thymus Gland - immunology</subject><ispartof>Clinical and experimental immunology, 1971-12, Vol.9 (6), p.839-851</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1713139/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1713139/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5316638$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Woodruff, M F</creatorcontrib><creatorcontrib>Inchley, M P</creatorcontrib><title>Cytolytic efficiency of rabbit-anti-mouse antilymphocytic globulin and its augmentation by antiglobulin</title><title>Clinical and experimental immunology</title><addtitle>Clin Exp Immunol</addtitle><description>The cytotoxic titre
−1
of rabbit-anti-mouse thymocyte IgG (ALG), determined by a modified test in which the cells were first incubated with ALG, then washed and re-incubated with guinea-pig C, ranged from 3110 to 5470. The cytolytic efficiency (i.e. the reciprocal of the mean number of IgG molecules attached per cell under conditions in which 50% lysis occurs in the presence of C), measured with
131
I-ALG, was about 0·002%. When γ
2
IgG from guinea-pigs immunized with rabbit IgG was added prior to the second incubation the cytotoxic titre was augmented up to ten-fold and the cytotoxic efficiency up to twenty-fold. The same γ
2
preparation resulted in up to 300-fold augmentation in a haemolytic system. In the thymocyte-ALG system, unlike the haemolytic system, augmentation was maximal at reduced concentrations of γ
2
. The mechanism of augmentation by γ
2
has been investigated using
131
I-ALG and
125
I- γ
2
IgG.</description><subject>Animals</subject><subject>Antibodies, Anti-Idiotypic</subject><subject>Antilymphocyte Serum</subject><subject>Chromatography, DEAE-Cellulose</subject><subject>Complement System Proteins</subject><subject>Cytotoxicity Tests, Immunologic</subject><subject>Goats</subject><subject>Guinea Pigs</subject><subject>Immunization</subject><subject>Immunoelectrophoresis</subject><subject>Immunoglobulin G</subject><subject>Iodine Isotopes</subject><subject>Mice</subject><subject>Rabbits</subject><subject>Thymus Gland - cytology</subject><subject>Thymus Gland - immunology</subject><issn>0009-9104</issn><issn>1365-2249</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1971</creationdate><recordtype>article</recordtype><recordid>eNpVkN1KxDAQhYMoa119BCEvEMhPkzY3ghR_Fha80esyaZNupG1Kmwp9e-u6il7NDOecj8OcoYQJJQnnqT5HCaVUE81oeomupul9PZVSfIM2UjClRJ6gplhiaJfoK2yd85W3fbXg4PAIxvhIoI-edGGeLP5a26UbDqE6-ps2mLn1_SrU2McJw9x0to8QfeixWY6BH9M1unDQTvbmNLfo7fHhtXgm-5enXXG_JwNXLJLc6SplOpXMKaprozgXQLUEyyjNXS6ZzVPIaw6pA8eVEwaky2TNaOY4NWKL7r65w2w6W1drnxHachh9B-NSBvDlf6X3h7IJHyXLmGBCr4Dbv4Df5Olj4hNs92yN</recordid><startdate>197112</startdate><enddate>197112</enddate><creator>Woodruff, M F</creator><creator>Inchley, M P</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>5PM</scope></search><sort><creationdate>197112</creationdate><title>Cytolytic efficiency of rabbit-anti-mouse antilymphocytic globulin and its augmentation by antiglobulin</title><author>Woodruff, M F ; Inchley, M P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p261t-8f9c419451f609db6223a095ae1008f851e84a8d2a4faf26f3ba5f75d107f20b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1971</creationdate><topic>Animals</topic><topic>Antibodies, Anti-Idiotypic</topic><topic>Antilymphocyte Serum</topic><topic>Chromatography, DEAE-Cellulose</topic><topic>Complement System Proteins</topic><topic>Cytotoxicity Tests, Immunologic</topic><topic>Goats</topic><topic>Guinea Pigs</topic><topic>Immunization</topic><topic>Immunoelectrophoresis</topic><topic>Immunoglobulin G</topic><topic>Iodine Isotopes</topic><topic>Mice</topic><topic>Rabbits</topic><topic>Thymus Gland - cytology</topic><topic>Thymus Gland - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Woodruff, M F</creatorcontrib><creatorcontrib>Inchley, M P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Clinical and experimental immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Woodruff, M F</au><au>Inchley, M P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytolytic efficiency of rabbit-anti-mouse antilymphocytic globulin and its augmentation by antiglobulin</atitle><jtitle>Clinical and experimental immunology</jtitle><addtitle>Clin Exp Immunol</addtitle><date>1971-12</date><risdate>1971</risdate><volume>9</volume><issue>6</issue><spage>839</spage><epage>851</epage><pages>839-851</pages><issn>0009-9104</issn><eissn>1365-2249</eissn><abstract>The cytotoxic titre
−1
of rabbit-anti-mouse thymocyte IgG (ALG), determined by a modified test in which the cells were first incubated with ALG, then washed and re-incubated with guinea-pig C, ranged from 3110 to 5470. The cytolytic efficiency (i.e. the reciprocal of the mean number of IgG molecules attached per cell under conditions in which 50% lysis occurs in the presence of C), measured with
131
I-ALG, was about 0·002%. When γ
2
IgG from guinea-pigs immunized with rabbit IgG was added prior to the second incubation the cytotoxic titre was augmented up to ten-fold and the cytotoxic efficiency up to twenty-fold. The same γ
2
preparation resulted in up to 300-fold augmentation in a haemolytic system. In the thymocyte-ALG system, unlike the haemolytic system, augmentation was maximal at reduced concentrations of γ
2
. The mechanism of augmentation by γ
2
has been investigated using
131
I-ALG and
125
I- γ
2
IgG.</abstract><cop>England</cop><pmid>5316638</pmid><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0009-9104 |
| ispartof | Clinical and experimental immunology, 1971-12, Vol.9 (6), p.839-851 |
| issn | 0009-9104 1365-2249 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1713139 |
| source | Open Access: PubMed Central |
| subjects | Animals Antibodies, Anti-Idiotypic Antilymphocyte Serum Chromatography, DEAE-Cellulose Complement System Proteins Cytotoxicity Tests, Immunologic Goats Guinea Pigs Immunization Immunoelectrophoresis Immunoglobulin G Iodine Isotopes Mice Rabbits Thymus Gland - cytology Thymus Gland - immunology |
| title | Cytolytic efficiency of rabbit-anti-mouse antilymphocytic globulin and its augmentation by antiglobulin |
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