Impairment of intestinal glutathione synthesis in patients with inflammatory bowel disease

Background—Reactive oxygen species contribute to tissue injury in inflammatory bowel disease (IBD). The tripeptide glutathione (GSH) is the most important intracellular antioxidant. Aims—To investigate constituent amino acid plasma levels and the GSH redox status in different compartments in IBD wit...

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Bibliographic Details
Published in:Gut 1998-04, Vol.42 (4), p.485-492
Main Authors: Sido, B, Hack, V, Hochlehnert, A, Lipps, H, Herfarth, C, Dröge, W
Format: Article
Language:English
Subjects:
Adult
Aged
Amino acids
Amino Acids - blood
Antioxidants
Biological and medical sciences
Colitis, Ulcerative - metabolism
Colon
Crohn Disease - metabolism
Crohn’s disease
Enzymes
Erythrocytes - metabolism
Female
gamma-Glutamyltransferase - metabolism
Gastroenterology. Liver. Pancreas. Abdomen
Glutamate-Cysteine Ligase - metabolism
glutathione
Glutathione - biosynthesis
Glutathione - metabolism
Humans
Inflammation and Inflammatory Bowel Disease
Inflammatory bowel disease
Intestinal Mucosa - metabolism
Laboratories
Leukocytes, Mononuclear - metabolism
Male
Medical sciences
Metabolism
Middle Aged
mucosa
Muscle, Skeletal - metabolism
Nutrition research
Other diseases. Semiology
Oxidation-Reduction
Oxidative stress
Physiology
Rodents
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Studies
ulcerative colitis
γ-glutamylcysteine synthetase
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Summary:Background—Reactive oxygen species contribute to tissue injury in inflammatory bowel disease (IBD). The tripeptide glutathione (GSH) is the most important intracellular antioxidant. Aims—To investigate constituent amino acid plasma levels and the GSH redox status in different compartments in IBD with emphasis on intestinal GSH synthesis in Crohn’s disease. Methods—Precursor amino acid levels were analysed in plasma and intestinal mucosa. Reduced (rGSH) and oxidised glutathione (GSSG) were determined enzymatically in peripheral blood mononuclear cells (PBMC), red blood cells (RBC), muscle, and in non-inflamed and inflamed ileum mucosa. Mucosal enzyme activity of γ-glutamylcysteine synthetase (γGCS) and γ-glutamyl transferase (γGT) was analysed. Blood of healthy subjects and normal mucosa from a bowel segment resected for tumour growth were used as controls. Results—Abnormally low plasma cysteine and cystine levels were associated with inflammation in IBD (p
ISSN:0017-5749
1468-3288
1458-3288
DOI:10.1136/gut.42.4.485