Correlation of desensitisation of platelet activating factor (PAF) receptors with intensity of inflammation and intestinal PAF content during experimental ileitis in guinea pig

• Published in: Gut 1998-09, Vol.43 (3), p.356-364
• Main Authors: Jeanneton, O, Delvaux, M, Langlois, Y, Le Bars, P, Le Bars, J, Delisle, M B, Frexinos, J, Bueno, L
• Format: Article
• Language: English
• Subjects: Acetylcholine - pharmacology; Animals; Biological and medical sciences; Cell Size - drug effects; Cholecystokinin - pharmacology; Crohn's disease; Dinoprostone - metabolism; Dinoprostone - pharmacology; Dose-Response Relationship, Drug; Enzymes; Experiments; Gastroenterology. Liver. Pancreas. Abdomen; Guinea Pigs; Ileitis - immunology; Ileitis - metabolism; Ileitis - pathology; Ileum - immunology; Ileum - metabolism; Ileum - pathology; Inflammation; Inflammation and Inflammatory Bowel Disease; Inflammatory bowel disease; intestinal inflammation; Male; Medical sciences; Motility; Muscle Contraction - drug effects; Muscle, Smooth - drug effects; Other diseases. Semiology; Platelet Activating Factor - analysis; Platelet Activating Factor - metabolism; Platelet Activating Factor - pharmacology; platelet activating factor receptor; Platelet Membrane Glycoproteins - drug effects; Platelet Membrane Glycoproteins - metabolism; prostaglandin E2 receptor; receptor desensitisation; Receptors, Cell Surface; Receptors, G-Protein-Coupled; Receptors, Prostaglandin E - drug effects; Receptors, Prostaglandin E - metabolism; Rodents; Smooth muscle; smooth muscle cells; Stimulation, Chemical; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus; Time Factors; Trinitrobenzenesulfonic Acid; trinitrobenzensulphonic acid
• ISSN: 0017-5749; 1468-3288; 1458-3288
• DOI: 10.1136/gut.43.3.356

Aim—To determine the kinetics of platelet activating factor (PAF) and prostaglandin E2 (PGE2) receptor desensitisation during intestinal inflammation induced by trinitrobenzenesulphonic acid (TNB) instillation and to study the relation between receptor regulation, inflammatory lesions, and PAF content of the gut wall. Methods—Receptor desensitisation was assessed on isolated smooth muscle cells from the circular layer. PAF content of the intestinal wall was determined by thin layer chromatography and radioimmunoassay. Results—After an acute inflammatory phase on day 1, subacute changes appeared in TNB instilled ileum, with a maximal intensity on day 6. In control animals, PAF 10 nM and PGE2 10 nM provoked a maximal contraction in the range of 24% of cell shortening. On days 1 and 3 after intestinal instillation of TNB, PAF induced contraction was not altered whereas the effect of PGE2 was progressively desensitised (2 logM rightward shift of its concentration-response curve: Cmax = 1 μM; p