Radiation induced cytochrome c release causes loss of rat colonic fluid absorption by damage to crypts and pericryptal myofibroblasts

• Published in: Gut 2000-11, Vol.47 (5), p.675-684
• Main Authors: Thiagarajah, J R, Gourmelon, P, Griffiths, N M, Lebrun, F, Naftalin, R J, Pedley, K C
• Format: Article
• Language: English
• Subjects: Animals; Apoptosis; Biological and medical sciences; cadherin; Cadherins - metabolism; Caspase Inhibitors; Caspases - metabolism; Caspases - physiology; Cell adhesion & migration; Cell Adhesion - radiation effects; Cell physiology; Collagen - metabolism; Colon; Colon - physiology; Colon - radiation effects; Cytochrome; cytochrome c; Cytochrome c Group - metabolism; Cytochrome c Group - radiation effects; Dextrans - pharmacokinetics; Dose-Response Relationship, Radiation; Fibroblasts - metabolism; Fibroblasts - radiation effects; Fluorescein-5-isothiocyanate - pharmacokinetics; Fundamental and applied biological sciences. Psychology; Intestinal Absorption - physiology; Intestinal Absorption - radiation effects; Male; Microscopy, Confocal; Molecular and cellular biology; Molecular weight; myofibroblast; pericryptal sheath; radiation; Rats; Rats, Wistar; Rodents; Signal transduction; Stem cells
• ISSN: 0017-5749; 1468-3288; 1458-3288
• DOI: 10.1136/gut.47.5.675

BACKGROUND Therapeutic or accidental exposure to radiation commonly causes gastrointestinal disturbances, including diarrhoea. Rats subjected to whole body ionising radiation at a dose of 8 Gy lose their capacity to absorb fluid via the descending colon after four days. After seven days, fluid absorption recovers to control levels. AIMS To investigate the effect of ionising radiation on colonic permeability together with its effect on mitochondria dependent apoptotic signals and intercellular adhesion molecules. METHODS Rats were irradiated with doses of 0–12 Gy. Colonic permeability was measured by accumulation of fluorescein isothiocyanate (FITC) dextran in crypt lumens. Changes in levels of cytochrome c, caspase 3, E and OB cadherin, β-catenin smooth muscle actin, and collagen IV were assessed using immunocytochemistry with confocal microscopy. RESULTS Cytosolic cytochrome c increased after 8 Gy (t1/2 1.4 (0.6) hours) and peaked at approximately six hours. Caspase 3 increased more slowly, particularly in crypt epithelial cells (t1/2 57 (14.5) hours). Pericryptal myofibroblasts disintegrated within 24 hours as was evident from loss of OB cadherin and smooth muscle actin. This coincided with increased crypt permeability to dextran. Intercellular adhesion between crypt luminal cells was not lost until day 4 when both β-catenin and E-cadherin were minimal. The half maximal dose-response for these effects was in the range 2–4 Gy. Recovery of colonic transport was concurrent with recovery of pericryptal smooth muscle actin and OB cadherin. The pan caspase inhibitor Z-Val-Ala-Asp.fluoromethylketone (1 mg/kg per day) had a small effect in conserving the pericryptal sheath myofibroblasts and sheath permeability but had no systemic therapeutic effects. CONCLUSIONS These data suggest that radiation damage to the colon may be initiated by mitochondrial events. Loss of crypt fluid absorption and increased permeability coincided with decreased intercellular adhesion between crypt epithelial cells and loss of pericryptal sheath barrier function.