Method for the measurement of antioxidant activity in human fluids

Aim—To develop a new, simple, and cheap method for estimating antioxidant activity in human fluids. Methods—The assay measured the capacity of the biological fluids to inhibit the production of thiobarbituric acid reactive substances (TBARS) from sodium benzoate under the influence of the free oxyge...

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Bibliographic Details
Published in:Journal of clinical pathology 2001-05, Vol.54 (5), p.356-361
Main Authors: Koracevic, D, Koracevic, G, Djordjevic, V, Andrejevic, S, Cosic, V
Format: Article
Language:English
Subjects:
Analysis
antioxidant activity
Antioxidants
Antioxidants - metabolism
Biological and medical sciences
Blood
Body fluids
Body Fluids - metabolism
Calibration
Dialysis
Fluids
free oxygen radicals
Free radicals (Chemistry)
Free Radicals - pharmacology
human fluids
Humans
Indicators and Reagents
Investigative techniques, diagnostic techniques (general aspects)
Leukemia, Lymphocytic, Chronic, B-Cell - blood
Medical sciences
Methods
Miscellaneous. Technology
Myocardial Infarction - blood
Oxidation-Reduction
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Physiological aspects
Physiology
Reaction Time
Reference Values
Reproducibility of Results
Sodium
Sodium Benzoate - metabolism
Temperature
Thiobarbituric Acid Reactive Substances - metabolism
Time Factors
Tissue Preservation
Uric acid
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Summary:Aim—To develop a new, simple, and cheap method for estimating antioxidant activity in human fluids. Methods—The assay measured the capacity of the biological fluids to inhibit the production of thiobarbituric acid reactive substances (TBARS) from sodium benzoate under the influence of the free oxygen radicals derived from Fenton's reaction. A solution of 1 mmol/litre uric acid was used as standard. Results—The following mean (SD) antioxidative activities were found (as uric acid) in the various biological fluids: serum, 2.04 (0.20) mmol/litre; urine, 176.5 (25.6) μmol/litre; cerebrospinal fluid, 95.0 (26.9) μmol/litre; aqueous humour oculi, 61.25 (9.9) μmol/litre; saliva, 838.5 (48.2) μmol/litre; tears, 247.0 (17.0) μmol/litre; ascites fluid, 270.0 (63.3) μmol/litre; kidney cyst fluid, 387.1 (28.1) μmol/litre. Small samples of the biological material were needed for the analyses: 10 μl of serum and 50–100 μl of other body fluids. In the sera of 48 healthy individuals there was a significant positive correlation between values obtained with the Randox method (as a reference method) and the new method proposed here (correlation coefficient, 0.8728; mean difference between methods,
ISSN:0021-9746
1472-4146
DOI:10.1136/jcp.54.5.356