Expression of tenascin-C in aseptic loosening of total hip replacement

• Published in: Annals of the rheumatic diseases 1998-10, Vol.57 (10), p.619-623
• Main Authors: Konttinen, Yrjö T, Li, Tian-Fang, Michelsson, Oliver, Xu, Jing-Wen, Sorsa, Timo, Santavirta, Seppo, Imai, Shinji, Virtanen, Ismo
• Format: Article
• Language: English
• Subjects: Adhesives; Aged; Arthroplasty, Replacement, Hip; aseptic loosening; Biological and medical sciences; Cytokines; Enzymes; Extended Reports; Female; Fibroblasts; Fibrosis; Hip Joint - metabolism; Hip Joint - pathology; Hospitals; Humans; Immunoenzyme Techniques; Inflammation; Investigative techniques, diagnostic techniques (general aspects); Joint Capsule - metabolism; Joint Capsule - pathology; Laboratories; Male; Medical sciences; Middle Aged; Osteoarticular system. Muscles; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Prostheses; Prosthesis Failure; Reoperation; Synovial Membrane - metabolism; Synovial Membrane - pathology; tenascin; Tenascin - metabolism; total hip replacement; Transplants & implants; Wound healing
• ISSN: 0003-4967; 1468-2060
• DOI: 10.1136/ard.57.10.619

OBJECTIVE To assess if the bonding interlayer between the implant and bone in aseptic loosening of total hip replacement (THR) is qualitatively deteriorated by excessive accumulation of anti-adhesive glycoprotein, tenascin-C. METHODS Alkaline phosphatase-anti-alkaline phosphatase (APAAP) method was used for immunohistochemical staining of tenascin-C in interface tissue and control synovial tissue. RESULTS Tenascin-C was found to be a major component of the extracellular matrix at a hitherto unrecognised site, namely the synovial membrane-like interface tissue between implant and bone in aseptic loosening of THR. The overall tenascin-C staining (median score 4.0) was greatly increased in aseptic loosening compared with synovial membrane (median score 2.0; p