Differential association of polymorphisms in the TNFα region with psoriatic arthritis but not psoriasis

• Published in: Annals of the rheumatic diseases 2002-03, Vol.61 (3), p.213-218
• Main Authors: Höhler, T, Grossmann, S, Stradmann-Bellinghausen, B, Kaluza, W, Reuss, E, de Vlam, K, Veys, E, Märker-Hermann, E
• Format: Article
• Language: English
• Subjects: ankylosing spondylitis; Biological and medical sciences; Dermatology; Extended Report; Genetic aspects; Genetic polymorphisms; Health aspects; lipopolysaccharide; LPS; major histocompatibility complex; Medical sciences; MHC; odds ratio; PBMC; PCR; peripheral blood mononuclear cells; polymerase chain reaction; polymorphisms; PsA; psoriasis; Psoriasis. Parapsoriasis. Lichen; Psoriatic arthritis; relative risk; rheumatoid arthritis; TNFα; tumour necrosis factor α
• ISSN: 0003-4967; 1468-2060
• DOI: 10.1136/ard.61.3.213

Objective: To investigate the potential association of tumour necrosis factor α (TNFα) microsatellite and promoter alleles with psoriatic arthritis (PsA). Methods: DNA from 89 white patients with PsA, 65 patients with psoriasis, and 99 healthy white controls was investigated for two TNFα promoter (–238 and –308) and three microsatellite polymorphisms (TNFa, c, and d). Patients had previously been studied by serology for HLA class I antigens and by sequence-specific polymerase chain reaction for DRB1* alleles. In addition, TNFα production of Ficoll separated peripheral blood mononuclear cells (PBMC) into culture supernatants after stimulation with lipopolysaccharide, αCD3 antibodies, phytohaemagglutinin, and streptococcal superantigen C was determined. Results: A significant, HLA class I independent increase of the TNFa6c1d3 haplotype was found in the group with PsA but not among patients with psoriasis (32% v 8%, pc