Interleukin 1ß and tumour necrosis factor α secreting cells are increased in the peripheral blood of patients with primary Sjögren's syndrome

Methods: PBMC spontaneously secreting tumour necrosis factor α (TNFα), interleukin 1ß (IL1ß), and interleukin 6 (IL6) were assessed by enzyme linked immunospot (ELISPOT) analysis in a cohort of 31 patients with pSS fulfilling the modified European classification criteria. Nineteen healthy volunteers...

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Published in:Annals of the rheumatic diseases 2003-04, Vol.62 (4), p.359-362
Main Authors: Willeke, P, Schotte, H, Schluter, B, Erren, M, Becker, H, Dyong, A, Mickholz, E, Domschke, W, Gaubitz, M
Format: Article
Language:English
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Summary:Methods: PBMC spontaneously secreting tumour necrosis factor α (TNFα), interleukin 1ß (IL1ß), and interleukin 6 (IL6) were assessed by enzyme linked immunospot (ELISPOT) analysis in a cohort of 31 patients with pSS fulfilling the modified European classification criteria. Nineteen healthy volunteers served as controls. ELISPOT results were correlated with glandular and extraglandular manifestations and autoantibody titres—that is, rheumatoid factor (RF) isotypes, anti-Ro/SS-A, anti-La/SS-B as determined by an enzyme linked immunosorbent assay (ELISA) technique. Results: The number of TNFα and IL1ß secreting cells was significantly higher in patients with pSS than in controls. No differences were detected in the number of IL6 secreting PBMC. Patients with recurrent parotid swelling (RPS) had a significantly increased number of IL1ß secreting PBMC. Moreover, the number of IL1ß secreting PBMC correlated with the disease duration ( r s =0.479; p
ISSN:0003-4967
1468-2060
DOI:10.1136/ard.62.4.359