Detecting structural changes in early experimental osteoarthritis of tibial cartilage by microscopic magnetic resonance imaging and polarised light microscopy

• Published in: Annals of the rheumatic diseases 2004-06, Vol.63 (6), p.709-717
• Main Authors: Alhadlaq, H A, Xia, Y, Moody, J B, Matyas, J R
• Format: Article
• Language: English
• Subjects: ACL; Animals; anterior cruciate ligament; Anterior Cruciate Ligament - pathology; Arthritis; Biological and medical sciences; cartilage; Cartilage, Articular - pathology; Disease Models, Animal; Diseases of the osteoarticular system; Dogs; Elasticity; Extended Report; Hindlimb; Knee; Ligaments; magnetic resonance imaging; Magnetic Resonance Imaging - methods; Medical sciences; microscopic magnetic resonance imaging; Microscopy, Polarization - methods; Miscellaneous. Osteoarticular involvement in other diseases; NMR; Nuclear magnetic resonance; Osteoarthritis; Osteoarthritis - pathology; PLM; polarised light microscopy; region of interest; ROI; Stress, Mechanical; Studies; T2 relaxation; Tibia; μMRI
• ISSN: 0003-4967; 1468-2060
• DOI: 10.1136/ard.2003.011783

Objectives: To detect changes in the collagen fibril network in articular cartilage in a canine experimental model of early osteoarthritis (OA) using microscopic magnetic resonance imaging (μMRI) and polarised light microscopy (PLM). Methods: Eighteen specimens from three pairs of the medial tibia of an anterior cruciate ligament transection canine model were subjected to μMRI and PLM study 12 weeks after surgery. For each specimen, the following experiments were carried out: (a) two dimensional μMRI images of T2 relaxation at four orientations; (b) the tangent Young’s modulus; and (c) two dimensional PLM images of optical retardance and fibril angle. Disease induced changes in tissue were examined across the depth of the cartilage at a μMRI resolution of 13.7–23.1 μm. Results: Several distinct changes from T2 weighted images of cartilage in OA tibia were seen. For the specimens that were covered at least in part by the meniscus, the significant changes in μMRI included a clear shift in the depth of maximum T2 (21–36%), a decrease in the superficial zone thickness (37–38%), and an increase in cartilage total thickness (15–27%). These μMRI changes varied topographically in the tibia surface because they were not significant in completely exposed locations in medial tibia. The μMRI results were confirmed by the PLM measurements and correlated well with the mechanical measurements. Conclusion: Both μMRI and PLM can detect quantitatively changes in collagen fibre architecture in early OA and resolve topographical variations in cartilage microstructure of canine tibia.