Loading…
The three-dimensional structure of complex I from Yarrowia lipolytica: A highly dynamic enzyme
The structure of complex I from Yarrowia lipolytica was determined by three-dimensional electron microscopy. A random conical data set was collected from deep stain embedded particles. More than 14 000 image pairs were analyzed. Through extensive classification combined with three-dimensional recons...
Saved in:
| Published in: | Journal of structural biology 2006-06, Vol.154 (3), p.269-279 |
|---|---|
| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c449t-2964e4d9f0bf49ea33b835735aa96c3480cdd22a9ba7684c9bfd0d681227947f3 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c449t-2964e4d9f0bf49ea33b835735aa96c3480cdd22a9ba7684c9bfd0d681227947f3 |
| container_end_page | 279 |
| container_issue | 3 |
| container_start_page | 269 |
| container_title | Journal of structural biology |
| container_volume | 154 |
| creator | Radermacher, M. Ruiz, T. Clason, T. Benjamin, S. Brandt, U. Zickermann, V. |
| description | The structure of complex I from
Yarrowia lipolytica was determined by three-dimensional electron microscopy. A random conical data set was collected from deep stain embedded particles. More than 14
000 image pairs were analyzed. Through extensive classification combined with three-dimensional reconstruction, it was possible for the first time to show a much more detailed substructure of the complex. The peripheral arm is subdivided in at least six domains. The membrane arm shows two major protrusions on its matrix facing side and exhibits a channel like feature on the side facing the cytoplasm. Structures resembling a tether connecting the subunits near the catalytic center with the protrusions of the membrane arm provide a second connection between matrix and membrane domain. |
| doi_str_mv | 10.1016/j.jsb.2006.02.011 |
| format | article |
| fullrecord | <record><control><sourceid>pubmed_cross</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1764498</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1047847706000670</els_id><sourcerecordid>16621601</sourcerecordid><originalsourceid>FETCH-LOGICAL-c449t-2964e4d9f0bf49ea33b835735aa96c3480cdd22a9ba7684c9bfd0d681227947f3</originalsourceid><addsrcrecordid>eNp9kMFq3DAQhkVp6G6SPkAvRS9gR5K1kpVAYQltEljIZXPIJUKWxrEW21okb1rn6eNll6S99DQD8___zHwIfaMkp4SKi02-SVXOCBE5YTmh9BOaU6IWWSkW8vO-5zIruZQzdJrShhDCKaNf0IwKwaggdI6e1g3goYkAmfMd9MmH3rQ4DXFnh10EHGpsQ7dt4Q--w3UMHX40MYbf3uDWb0M7Dt6aS7zEjX9u2hG7sTedtxj617GDc3RSmzbB12M9Qw-_fq6vb7PV_c3d9XKVWc7VkDElOHCnalLVXIEpiqosFrJYGKOELXhJrHOMGVUZKUpuVVU74kRJGZOKy7o4Qz8Oudtd1YGz0A_RtHobfWfiqIPx-t9J7xv9HF40lWK6oJwC6CHAxpBShPrdS4new9YbPcHWe9iaMD3Bnjzf_1764TjSnQRXBwFMr794iDpZD70F5yPYQbvg_xP_Bi0wkhY</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>The three-dimensional structure of complex I from Yarrowia lipolytica: A highly dynamic enzyme</title><source>ScienceDirect Journals</source><creator>Radermacher, M. ; Ruiz, T. ; Clason, T. ; Benjamin, S. ; Brandt, U. ; Zickermann, V.</creator><creatorcontrib>Radermacher, M. ; Ruiz, T. ; Clason, T. ; Benjamin, S. ; Brandt, U. ; Zickermann, V.</creatorcontrib><description>The structure of complex I from
Yarrowia lipolytica was determined by three-dimensional electron microscopy. A random conical data set was collected from deep stain embedded particles. More than 14
000 image pairs were analyzed. Through extensive classification combined with three-dimensional reconstruction, it was possible for the first time to show a much more detailed substructure of the complex. The peripheral arm is subdivided in at least six domains. The membrane arm shows two major protrusions on its matrix facing side and exhibits a channel like feature on the side facing the cytoplasm. Structures resembling a tether connecting the subunits near the catalytic center with the protrusions of the membrane arm provide a second connection between matrix and membrane domain.</description><identifier>ISSN: 1047-8477</identifier><identifier>EISSN: 1095-8657</identifier><identifier>DOI: 10.1016/j.jsb.2006.02.011</identifier><identifier>PMID: 16621601</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>3D electron microscopy ; 3D reconstruction ; Cell Membrane - metabolism ; Complex I ; Computational Biology - methods ; Cytoplasm - metabolism ; Electron Transport Complex I - chemistry ; Image processing ; Image Processing, Computer-Assisted ; Microscopy, Electron ; Mitochondria ; Molecular Conformation ; NADH-dehydrogenase ; Protein Conformation ; Protein Structure, Tertiary ; Radon - chemistry ; Radon transform ; Structure ; Yarrowia - enzymology ; Yarrowia lipolytica</subject><ispartof>Journal of structural biology, 2006-06, Vol.154 (3), p.269-279</ispartof><rights>2006 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c449t-2964e4d9f0bf49ea33b835735aa96c3480cdd22a9ba7684c9bfd0d681227947f3</citedby><cites>FETCH-LOGICAL-c449t-2964e4d9f0bf49ea33b835735aa96c3480cdd22a9ba7684c9bfd0d681227947f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16621601$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Radermacher, M.</creatorcontrib><creatorcontrib>Ruiz, T.</creatorcontrib><creatorcontrib>Clason, T.</creatorcontrib><creatorcontrib>Benjamin, S.</creatorcontrib><creatorcontrib>Brandt, U.</creatorcontrib><creatorcontrib>Zickermann, V.</creatorcontrib><title>The three-dimensional structure of complex I from Yarrowia lipolytica: A highly dynamic enzyme</title><title>Journal of structural biology</title><addtitle>J Struct Biol</addtitle><description>The structure of complex I from
Yarrowia lipolytica was determined by three-dimensional electron microscopy. A random conical data set was collected from deep stain embedded particles. More than 14
000 image pairs were analyzed. Through extensive classification combined with three-dimensional reconstruction, it was possible for the first time to show a much more detailed substructure of the complex. The peripheral arm is subdivided in at least six domains. The membrane arm shows two major protrusions on its matrix facing side and exhibits a channel like feature on the side facing the cytoplasm. Structures resembling a tether connecting the subunits near the catalytic center with the protrusions of the membrane arm provide a second connection between matrix and membrane domain.</description><subject>3D electron microscopy</subject><subject>3D reconstruction</subject><subject>Cell Membrane - metabolism</subject><subject>Complex I</subject><subject>Computational Biology - methods</subject><subject>Cytoplasm - metabolism</subject><subject>Electron Transport Complex I - chemistry</subject><subject>Image processing</subject><subject>Image Processing, Computer-Assisted</subject><subject>Microscopy, Electron</subject><subject>Mitochondria</subject><subject>Molecular Conformation</subject><subject>NADH-dehydrogenase</subject><subject>Protein Conformation</subject><subject>Protein Structure, Tertiary</subject><subject>Radon - chemistry</subject><subject>Radon transform</subject><subject>Structure</subject><subject>Yarrowia - enzymology</subject><subject>Yarrowia lipolytica</subject><issn>1047-8477</issn><issn>1095-8657</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNp9kMFq3DAQhkVp6G6SPkAvRS9gR5K1kpVAYQltEljIZXPIJUKWxrEW21okb1rn6eNll6S99DQD8___zHwIfaMkp4SKi02-SVXOCBE5YTmh9BOaU6IWWSkW8vO-5zIruZQzdJrShhDCKaNf0IwKwaggdI6e1g3goYkAmfMd9MmH3rQ4DXFnh10EHGpsQ7dt4Q--w3UMHX40MYbf3uDWb0M7Dt6aS7zEjX9u2hG7sTedtxj617GDc3RSmzbB12M9Qw-_fq6vb7PV_c3d9XKVWc7VkDElOHCnalLVXIEpiqosFrJYGKOELXhJrHOMGVUZKUpuVVU74kRJGZOKy7o4Qz8Oudtd1YGz0A_RtHobfWfiqIPx-t9J7xv9HF40lWK6oJwC6CHAxpBShPrdS4new9YbPcHWe9iaMD3Bnjzf_1764TjSnQRXBwFMr794iDpZD70F5yPYQbvg_xP_Bi0wkhY</recordid><startdate>20060601</startdate><enddate>20060601</enddate><creator>Radermacher, M.</creator><creator>Ruiz, T.</creator><creator>Clason, T.</creator><creator>Benjamin, S.</creator><creator>Brandt, U.</creator><creator>Zickermann, V.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>20060601</creationdate><title>The three-dimensional structure of complex I from Yarrowia lipolytica: A highly dynamic enzyme</title><author>Radermacher, M. ; Ruiz, T. ; Clason, T. ; Benjamin, S. ; Brandt, U. ; Zickermann, V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c449t-2964e4d9f0bf49ea33b835735aa96c3480cdd22a9ba7684c9bfd0d681227947f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>3D electron microscopy</topic><topic>3D reconstruction</topic><topic>Cell Membrane - metabolism</topic><topic>Complex I</topic><topic>Computational Biology - methods</topic><topic>Cytoplasm - metabolism</topic><topic>Electron Transport Complex I - chemistry</topic><topic>Image processing</topic><topic>Image Processing, Computer-Assisted</topic><topic>Microscopy, Electron</topic><topic>Mitochondria</topic><topic>Molecular Conformation</topic><topic>NADH-dehydrogenase</topic><topic>Protein Conformation</topic><topic>Protein Structure, Tertiary</topic><topic>Radon - chemistry</topic><topic>Radon transform</topic><topic>Structure</topic><topic>Yarrowia - enzymology</topic><topic>Yarrowia lipolytica</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Radermacher, M.</creatorcontrib><creatorcontrib>Ruiz, T.</creatorcontrib><creatorcontrib>Clason, T.</creatorcontrib><creatorcontrib>Benjamin, S.</creatorcontrib><creatorcontrib>Brandt, U.</creatorcontrib><creatorcontrib>Zickermann, V.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of structural biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Radermacher, M.</au><au>Ruiz, T.</au><au>Clason, T.</au><au>Benjamin, S.</au><au>Brandt, U.</au><au>Zickermann, V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The three-dimensional structure of complex I from Yarrowia lipolytica: A highly dynamic enzyme</atitle><jtitle>Journal of structural biology</jtitle><addtitle>J Struct Biol</addtitle><date>2006-06-01</date><risdate>2006</risdate><volume>154</volume><issue>3</issue><spage>269</spage><epage>279</epage><pages>269-279</pages><issn>1047-8477</issn><eissn>1095-8657</eissn><abstract>The structure of complex I from
Yarrowia lipolytica was determined by three-dimensional electron microscopy. A random conical data set was collected from deep stain embedded particles. More than 14
000 image pairs were analyzed. Through extensive classification combined with three-dimensional reconstruction, it was possible for the first time to show a much more detailed substructure of the complex. The peripheral arm is subdivided in at least six domains. The membrane arm shows two major protrusions on its matrix facing side and exhibits a channel like feature on the side facing the cytoplasm. Structures resembling a tether connecting the subunits near the catalytic center with the protrusions of the membrane arm provide a second connection between matrix and membrane domain.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>16621601</pmid><doi>10.1016/j.jsb.2006.02.011</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1047-8477 |
| ispartof | Journal of structural biology, 2006-06, Vol.154 (3), p.269-279 |
| issn | 1047-8477 1095-8657 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1764498 |
| source | ScienceDirect Journals |
| subjects | 3D electron microscopy 3D reconstruction Cell Membrane - metabolism Complex I Computational Biology - methods Cytoplasm - metabolism Electron Transport Complex I - chemistry Image processing Image Processing, Computer-Assisted Microscopy, Electron Mitochondria Molecular Conformation NADH-dehydrogenase Protein Conformation Protein Structure, Tertiary Radon - chemistry Radon transform Structure Yarrowia - enzymology Yarrowia lipolytica |
| title | The three-dimensional structure of complex I from Yarrowia lipolytica: A highly dynamic enzyme |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T09%3A46%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20three-dimensional%20structure%20of%20complex%20I%20from%20Yarrowia%20lipolytica:%20A%20highly%20dynamic%20enzyme&rft.jtitle=Journal%20of%20structural%20biology&rft.au=Radermacher,%20M.&rft.date=2006-06-01&rft.volume=154&rft.issue=3&rft.spage=269&rft.epage=279&rft.pages=269-279&rft.issn=1047-8477&rft.eissn=1095-8657&rft_id=info:doi/10.1016/j.jsb.2006.02.011&rft_dat=%3Cpubmed_cross%3E16621601%3C/pubmed_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c449t-2964e4d9f0bf49ea33b835735aa96c3480cdd22a9ba7684c9bfd0d681227947f3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/16621601&rfr_iscdi=true |