A Droplet-Based, Composite PDMS/Glass Capillary Microfluidic System for Evaluating Protein Crystallization Conditions by Microbatch and Vapor-Diffusion Methods with On-Chip X-Ray Diffraction

On‐chip protein crystallization and diffraction: Proteins can be crystallized in nanoliter volumes by using both microbatch and vapor‐diffusion techniques inside composite PDMS/glass microfluidic devices (PDMS=polydimethylsiloxane). The quality of crystals can be assessed directly by on‐chip X‐ray d...

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Published in:Angewandte Chemie International Edition 2004-05, Vol.43 (19), p.2508-2511
Main Authors: Zheng, Bo, Tice, Joshua D., Roach, L. Spencer, Ismagilov, Rustem F.
Format: Article
Language:English
Subjects:
crystal growth
Crystallization - instrumentation
Crystallization - methods
Crystallography, X-Ray - methods
Diffusion
Dimethylpolysiloxanes - chemistry
Fluorocarbons - chemistry
Glass - chemistry
Microfluidics
microreactors
Oils - chemistry
Plant Proteins - chemistry
proteins
Proteins - chemistry
Siloxanes - chemistry
Sodium Chloride - chemistry
Water - chemistry
X-ray diffraction
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cited_by cdi_FETCH-LOGICAL-c4744-2548fe6a877b6ef20e4c1fbf0afadc51ada365e96976b28ec9ceeaf9f21430f53
cites cdi_FETCH-LOGICAL-c4744-2548fe6a877b6ef20e4c1fbf0afadc51ada365e96976b28ec9ceeaf9f21430f53
container_end_page 2511
container_issue 19
container_start_page 2508
container_title Angewandte Chemie International Edition
container_volume 43
creator Zheng, Bo
Tice, Joshua D.
Roach, L. Spencer
Ismagilov, Rustem F.
description On‐chip protein crystallization and diffraction: Proteins can be crystallized in nanoliter volumes by using both microbatch and vapor‐diffusion techniques inside composite PDMS/glass microfluidic devices (PDMS=polydimethylsiloxane). The quality of crystals can be assessed directly by on‐chip X‐ray diffraction (see picture).
doi_str_mv 10.1002/anie.200453974
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source Wiley-Blackwell Read & Publish Collection
subjects crystal growth
Crystallization - instrumentation
Crystallization - methods
Crystallography, X-Ray - methods
Diffusion
Dimethylpolysiloxanes - chemistry
Fluorocarbons - chemistry
Glass - chemistry
Microfluidics
microreactors
Oils - chemistry
Plant Proteins - chemistry
proteins
Proteins - chemistry
Siloxanes - chemistry
Sodium Chloride - chemistry
Water - chemistry
X-ray diffraction
title A Droplet-Based, Composite PDMS/Glass Capillary Microfluidic System for Evaluating Protein Crystallization Conditions by Microbatch and Vapor-Diffusion Methods with On-Chip X-Ray Diffraction
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