The relation between bone marrow angiogenesis and the proliferation index Ki-67 in multiple myeloma

• Published in: Journal of clinical pathology 2004-08, Vol.57 (8), p.856-860
• Main Authors: Alexandrakis, M G, Passam, F H, Dambaki, C, Pappa, C A, Stathopoulos, E N
• Format: Article
• Language: English
• Subjects: Acute Disease; ADP-ribosyl Cyclase - analysis; ADP-ribosyl Cyclase 1; Adult; Aged; Aged, 80 and over; alkaline–antialkaline phosphatase; Analysis of Variance; Angiogenesis; Antigens, CD - analysis; APAAP; Biological and medical sciences; Biomarkers - analysis; Biopsy; Bone marrow; Bone Marrow Cells - pathology; C-Reactive Protein - analysis; Cell cycle; Cell Division; Chemotherapy; CRP; C reactive protein; Female; Hematologic and hematopoietic diseases; Hematology; Humans; Immunodeficiencies. Immunoglobulinopathies; Immunoglobulinopathies; Immunohistochemistry - methods; Immunopathology; Investigative techniques, diagnostic techniques (general aspects); Ki-67; Ki-67 Antigen - analysis; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis; Lymphoma; Male; Medical prognosis; Medical sciences; Membrane Glycoproteins; microvascular density; Middle Aged; Multiple myeloma; Multiple Myeloma - drug therapy; Multiple Myeloma - pathology; MVD; Neovascularization, Pathologic - pathology; Original; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Phosphatase; Plasma; Prognosis; proliferation index; Statistics, Nonparametric; Studies; TBS; Tris buffered saline; Tumors
• ISSN: 0021-9746; 1472-4146
• DOI: 10.1136/jcp.2003.013110

Aim: Angiogenesis correlates with disease progression in various haematological malignancies. This study investigated the association between microvascular density (MVD) and the Ki-67 proliferation index (Ki-67 PI), bone marrow infiltration, and C reactive protein (CRP) in patients with multiple myeloma. Methods: Bone marrow MVD was examined in 44 biopsies at diagnosis and 15 in plateau phase by immunostaining the endothelial cells with a monoclonal antibody to CD34. The Ki-67 PI was evaluated by a double immunostaining technique using the monoclonal antibodies MIB-1 and CD38. Results: MVD, Ki-67 PI, bone marrow infiltration, and CRP were significantly higher in pretreatment patients than in controls and decreased in patients achieving plateau phase. MVD significantly correlated with Ki-67 PI and infiltration, and Ki-67 correlated with infiltration. Conclusion: In multiple myeloma, apart from being a marker of proliferative activity, Ki-67 is also associated with bone marrow angiogenesis and tumour burden.