MUC4 is increased in high grade intraepithelial neoplasia in Barrett’s oesophagus and is associated with a proapoptotic Bax to Bcl-2 ratio

Background: Patients with Barrett’s oesophagus (BO) are at risk of oesophageal adenocarcinoma. Because the pattern of mucosal mucins changes during neoplastic progression, it may serve as a marker of intraepithelial neoplasia. Aims: To determine the expression pattern of mucins in neoplastic BO epit...

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Bibliographic Details
Published in:Journal of clinical pathology 2004-12, Vol.57 (12), p.1267-1272
Main Authors: Bax, D A, Haringsma, J, Einerhand, A W C, van Dekken, H, Blok, P, Siersema, P D, Kuipers, E J, Kusters, J G
Format: Article
Language:English
Subjects:
ADC
adenocarcinoma
Adenocarcinoma - chemistry
alkaline phosphatase
Apoptosis
Apoptosis - physiology
Barrett Esophagus - metabolism
Barrett’s oesophagus
bcl-2-Associated X Protein
Biopsy
Carcinoma in Situ - chemistry
Cell adhesion & migration
Cell cycle
Endoscopy
Esophageal Neoplasms - chemistry
Gene Expression Regulation, Neoplastic
HGN
high grade dysplasia
high grade intraepithelial neoplasia
Humans
Hypotheses
Ligands
Medical prognosis
Mucin-4
mucins
Mucins - analysis
Neoplasm Proteins - analysis
Original
PCR
polymerase chain reaction
Proteins
Proto-Oncogene Proteins c-bcl-2 - analysis
Reverse Transcriptase Polymerase Chain Reaction - methods
reverse transcription
RNA, Messenger - analysis
RNA, Neoplasm - analysis
Studies
Surveillance
Tumors
tumour markers
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Summary:Background: Patients with Barrett’s oesophagus (BO) are at risk of oesophageal adenocarcinoma. Because the pattern of mucosal mucins changes during neoplastic progression, it may serve as a marker of intraepithelial neoplasia. Aims: To determine the expression pattern of mucins in neoplastic BO epithelium (high grade dysplasia) and correlate it with the expression of apoptosis markers Bax and Bcl-2. Methods: Thirty seven patients with BO were studied: 16 without intraepithelial neoplasia, six with high grade intraepithelial neoplasia (HGN), and 15 with infiltrating adenocarcinoma. Biopsies were obtained from squamous epithelium, Barrett’s epithelium, and (when present) foci of suspected HGN or adenocarcinoma. MUC1–4, MUC5AC, MUC5B, MUC6, Bax, and Bcl-2 mRNA were determined by semiquantitative RT-PCR. MUC2, MUC5AC, and MUC6 protein was determined by immunoblotting. Results: Mucin expression varied between neoplastic progression stages in BO. Mucin mRNA levels were low in squamous epithelium, except for MUC4, and were at least four times higher in BO and HGN (p
ISSN:0021-9746
1472-4146
DOI:10.1136/jcp.2004.017020