Deposition of silicone oil droplets in the residual anterior lens capsule after vitrectomy and lensectomy in rabbits

• Published in: British journal of ophthalmology 2004-05, Vol.88 (5), p.703-707
• Main Authors: Miyamoto, T, Saika, S, Yamanaka, A, Okada, Y, Ohnishi, Y
• Format: Article
• Language: English
• Subjects: Animals; anterior capsule; Biological and medical sciences; Biotechnology; Cataracts; Collagen; Collagen - metabolism; Diabetic retinopathy; Emulsions; Endothelium; Epithelial Cells - ultrastructure; Extracellular matrix; Extracellular Matrix - metabolism; Fibroblasts; Fibronectins - metabolism; Histology; Laboratory Science - Extended Reports; Lens Capsule, Crystalline - metabolism; Lens Capsule, Crystalline - ultrastructure; Lens, Crystalline - surgery; lensectomy; Medical sciences; Microscopy, Electron; opacification; pars plana lensectomy; PEA; phacoemulsification and aspiration; PPL; Pressure; Rabbits; silicone oil; Silicone Oils - pharmacokinetics; Smooth muscle; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Surgery of the eye and orbit; Vitrectomy; Wound Healing - physiology
• ISSN: 0007-1161; 1468-2079
• DOI: 10.1136/bjo.2003.021113

Aim: To examine the histology of preserved anterior lens capsule in vitrectomised and lensectomised rabbit eyes with and without silicone oil tamponade. Methods: Forty adult Japanese albino rabbits received two port vitrectomy and lensectomy with or without silicone oil tamponade in one eye under both general and topical anaesthesia. Anterior lens capsule was preserved during operation. After healing intervals residual anterior capsule was histologically observed under light or electron microscopy. Results: Immediately after operation, cuboidal lens epithelial cells were observed on the posterior surface of the preserved anterior capsule. During healing intervals in eyes with or without silicone oil tamponade, regenerated lens structure of Sommerring’s ring and fibrous tissue formed in the peripheral and central areas of the residual capsule, respectively. Ultrastructural observation revealed the presence of many vacuoles amid matrix accumulation on the posterior capsular surface, suggesting the deposition of emulsified silicone oil droplets. Conclusion: Lens epithelial cells produce regenerated lenticular structure and fibrous tissue on the residual capsule following vitrectomy and lensectomy in rabbits. Silicone oil droplets formed by its emulsification deposit in extracellular matrix accumulated on the posterior surface of the anterior capsule. Emulsified silicone may potentially enhance opacification of residual anterior capsule following pars plana vitrectomy by silicone oil deposition and subsequent activation of lens epithelial cells.