MMP inhibition prevents human lens epithelial cell migration and contraction of the lens capsule

Purpose: The development of posterior capsule contraction following cataract surgery is caused by the activity of residual lens epithelial cells. Matrix metalloproteinases (MMPs) are a group of proteolytic enzymes, which are essential for cell migration and cell mediated contraction following wound...

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Bibliographic Details
Published in:British journal of ophthalmology 2004-07, Vol.88 (7), p.868-872
Main Authors: Wong, T T L, Daniels, J T, Crowston, J G, Khaw, P T
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cataract Extraction - adverse effects
Cataracts
Cell adhesion & migration
Cell Movement - drug effects
Cell Movement - physiology
Cell Survival - physiology
Clinical Science - Scientific Reports
Collagen
DMEM
Dulbecco’s modified Eagle’s medium
Enzymes
Epithelial Cells - drug effects
Epithelial Cells - physiology
Eye surgery
FCS
fetal calf serum
Fibroblasts
Humans
Hydroxamic Acids
Indoles - pharmacology
Laboratories
LECs
Lens Capsule, Crystalline - cytology
Lens Capsule, Crystalline - drug effects
lens epithelial cells
Lens, Crystalline - cytology
matrix contraction
matrix metalloproteinase
Matrix Metalloproteinase 2 - biosynthesis
Matrix Metalloproteinase 9 - biosynthesis
Matrix Metalloproteinase Inhibitors
Medical sciences
MMP
PBS
PCO
phosphate buffered saline
posterior capsule opacification
Studies
Surgery
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Surgery of the eye and orbit
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Summary:Purpose: The development of posterior capsule contraction following cataract surgery is caused by the activity of residual lens epithelial cells. Matrix metalloproteinases (MMPs) are a group of proteolytic enzymes, which are essential for cell migration and cell mediated contraction following wound healing. The authors investigated whether inhibiting MMP activity can reduce lens epithelial cell migration and as a result, lead to a reduction in cell mediated capsule contraction. Methods: Human donor lens capsules were cultured and treated with a broad spectrum MMP inhibitor, Ilomastat (GM6001). MMP-2 and MMP-9 production were determined by ELISA. Cell migration onto the posterior capsule and capsule contraction were digitally measured. Results: MMP inhibition significantly reduced lens epithelial cell migration onto the posterior capsule (p
ISSN:0007-1161
1468-2079
DOI:10.1136/bjo.2003.034629