Effect of physiological concentrations of vitamin C on gastric cancer cells and Helicobacter pylori

Background: Gastric juice vitamin C may be protective against gastric carcinogenesis but concentrations are significantly reduced by Helicobacter pylori infection. We investigated the in vitro effects of vitamin C at concentrations comparable with those found in gastric juice on gastric cancer cells...

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Bibliographic Details
Published in:Gut 2002-02, Vol.50 (2), p.165-169
Main Authors: Zhang, Z-W, Abdullahi, M, Farthing, M J G
Format: Article
Language:English
Subjects:
Adenocarcinoma - pathology
Adenocarcinoma - prevention & control
Apoptosis
Ascorbic Acid - physiology
Bacterial Adhesion - physiology
Biological and medical sciences
BrdU
bromodeoxyuridine
Cell Division
Cell growth
dehydroascobic acid
DHA
FITC
Flow Cytometry - methods
fluorescein isothiocyanate
gastric cancer
Gastric Juice - chemistry
Gastroenterology. Liver. Pancreas. Abdomen
Helicobacter infections
Helicobacter Infections - prevention & control
Helicobacter Pylori
Helicobacter pylori - growth & development
Helicobacter pylori - pathogenicity
Helicobacter pylori - physiology
Hemagglutination - physiology
Hemagglutination Tests
Humans
Hydrogen-Ion Concentration
Intestinal Mucosa - microbiology
Medical sciences
N-nitroso compounds
NOC
PBS
phosphate buffered saline
Physiological aspects
RBCs
reactive oxygen metabolites
red blood cells
ROM
Stomach Neoplasms - pathology
Stomach Neoplasms - prevention & control
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Studies
Tumor Cells, Cultured
Tumors
Vitamin C
Vitamin C metabolism
Vitamin metabolism
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Summary:Background: Gastric juice vitamin C may be protective against gastric carcinogenesis but concentrations are significantly reduced by Helicobacter pylori infection. We investigated the in vitro effects of vitamin C at concentrations comparable with those found in gastric juice on gastric cancer cells and H pylori. Methods: Gastric cancer cell lines and various H pylori strains were treated with l-ascorbic acid for up to 72 hours. Cell viability, and protein and DNA synthesis were determined. Flow cytometry was used for assessment of H pylori adherence, cell cycle distribution, and apoptosis. H pylori growth and its haemagglutination activity were determined using viability count and microtitration assay. Results: Vitamin C induced a significant dose dependent growth inhibition of gastric AGS and MKN45 cells but this effect was significantly reduced at levels similar to those in gastric juice of H pylori infected patients (
ISSN:0017-5749
1468-3288
1458-3288
DOI:10.1136/gut.50.2.165