Lactic acid bacteria secrete metabolites retaining anti-inflammatory properties after intestinal transport

• Published in: Gut 2004-06, Vol.53 (6), p.821-828
• Main Authors: Ménard, S, Candalh, C, Bambou, J C, Terpend, K, Cerf-Bensussan, N, Heyman, M
• Format: Article
• Language: English
• Subjects: Bacteria; Bb CM; Bifidobacterium - metabolism; Bifidobacterium breve conditioned media; Biological and medical sciences; Biological Transport; Cell Line; Culture Media, Conditioned - pharmacology; Cytokines; FCS; fetal calf serum; Flow cytometry; human serum AB; Humans; IEC; IFN; Inflammation; inflammatory cytokines; interferon; interleukin; Interleukin-10 - metabolism; intestinal barrier; intestinal epithelial cells; Intestinal Mucosa - metabolism; Intestine Inflammation; Intestines - microbiology; LBP; Leukocytes, Mononuclear - metabolism; lipopolysaccharide; Lipopolysaccharides - pharmacology; lipoteichoic acid; LPS; LPS binding protein; LTA; Malformations of the nervous system; Medical sciences; Metabolites; Neurology; NF-kappa B - metabolism; NFκB; nuclear factor κB; PBMC; peripheral blood mononuclear cells; Probiotics; SAB; St CM; Streptococcus - metabolism; Streptococcus thermophilus conditioned media; TGF-β; TLR; TNF-α; toll-like receptor; transforming growth factor β; Tumor Necrosis Factor-alpha - antagonists & inhibitors; Tumor Necrosis Factor-alpha - metabolism; Tumor necrosis factor-TNF; tumour necrosis factor α; Yogurt
• ISSN: 0017-5749; 1468-3288; 1458-3288
• DOI: 10.1136/gut.2003.026252

Background: Probiotic bacteria have a beneficial effect on intestinal inflammation. In this study, we have examined the effect of lactic acid and commensal Gram positive (+) bacteria conditioned media (CM) on tumour necrosis factor α (TNF-α) release and the mechanisms involved. Methods: Lipopolysaccharide (LPS) induced TNF-α secretion by peripheral blood mononuclear cells or the THP-1 cell line was monitored in the presence or absence of bacteria CM obtained from two probiotic strains, Bifidobacterium breve (Bb) and Streptococcus thermophilus (St), and three commensal bacterial strains (Bifidobacterium bifidum, Ruminococcus gnavus, and unidentified Streptococcus). Bb and St bacteria CM were allowed to cross filter grown intestinal epithelial cell monolayers (HT29-19A) to assess intestinal transport of active bacterial products. These products were characterised and their effect on LPS binding to THP-1 cells and nuclear factor κB (NFκB) activation assessed. Results: Dose dependent inhibition of LPS induced TNF-α secretion was noted for both probiotic bacteria CM (64% and 71% inhibition for Bb and St, respectively) and to a lesser extent commensal bacteria CM (21–32% inhibition). Active products from Bb and St were resistant to digestive enzymes and had a molecular mass