Defective denominators

[...]I would never recommend the use of proliferating cell nuclear antigen as a marker of cell proliferation as: (1) the method is difficult to standardise; (2) the antigen has a long half life; and (3) anomalous expression has been demonstrated in non-cycling near tumours and after administration o...

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Bibliographic Details
Published in:Gut 2005-10, Vol.54 (10), p.1502-1502; author reply 1503
Main Author: Goodlad, R A
Format: Article
Language:English
Subjects:
Antigens
Carbohydrates
Cell cycle
Cell growth
Cell Proliferation - drug effects
Colorectal cancer
defective denominators
Diet
Humans
Intestinal Mucosa - cytology
Intestinal Mucosa - microbiology
Labeling
large bowel
Letter
Microdissection - methods
microflora
Mitosis - physiology
Mitotic Index
Prebiotics
Probiotics - pharmacology
Proteins
Senescence
Studies
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Summary:[...]I would never recommend the use of proliferating cell nuclear antigen as a marker of cell proliferation as: (1) the method is difficult to standardise; (2) the antigen has a long half life; and (3) anomalous expression has been demonstrated in non-cycling near tumours and after administration of growth factors. 3 For sections, Ki67 is far better however even using this antibody the results of the present study are unlikely to be conclusive as only 2-4 crypts could be scored; for most studies I would recommend scoring 30 hemi crypts. [...]I think that the jury is still out on the "protective role" of fermentable non-starch polysaccharides (fibre) as while the EPIC study showed a dramatic effect of intrinsically high fibre diets, 3 many others have shown null effects and some of these, especially the intervention ones, demonstrated adverse effects.
ISSN:0017-5749
1468-3288