Defective denominators

[...]I would never recommend the use of proliferating cell nuclear antigen as a marker of cell proliferation as: (1) the method is difficult to standardise; (2) the antigen has a long half life; and (3) anomalous expression has been demonstrated in non-cycling near tumours and after administration o...

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Published in:Gut 2005-10, Vol.54 (10), p.1502-1502; author reply 1503
Main Author: Goodlad, R A
Format: Article
Language:English
Subjects:
Antigens
Carbohydrates
Cell cycle
Cell growth
Cell Proliferation - drug effects
Colorectal cancer
defective denominators
Diet
Humans
Intestinal Mucosa - cytology
Intestinal Mucosa - microbiology
Labeling
large bowel
Letter
Microdissection - methods
microflora
Mitosis - physiology
Mitotic Index
Prebiotics
Probiotics - pharmacology
Proteins
Senescence
Studies
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description [...]I would never recommend the use of proliferating cell nuclear antigen as a marker of cell proliferation as: (1) the method is difficult to standardise; (2) the antigen has a long half life; and (3) anomalous expression has been demonstrated in non-cycling near tumours and after administration of growth factors. 3 For sections, Ki67 is far better however even using this antibody the results of the present study are unlikely to be conclusive as only 2-4 crypts could be scored; for most studies I would recommend scoring 30 hemi crypts. [...]I think that the jury is still out on the "protective role" of fermentable non-starch polysaccharides (fibre) as while the EPIC study showed a dramatic effect of intrinsically high fibre diets, 3 many others have shown null effects and some of these, especially the intervention ones, demonstrated adverse effects.
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[...]I think that the jury is still out on the "protective role" of fermentable non-starch polysaccharides (fibre) as while the EPIC study showed a dramatic effect of intrinsically high fibre diets, 3 many others have shown null effects and some of these, especially the intervention ones, demonstrated adverse effects.</description><identifier>ISSN: 0017-5749</identifier><identifier>EISSN: 1468-3288</identifier><identifier>PMID: 16162959</identifier><identifier>CODEN: GUTTAK</identifier><language>eng</language><publisher>England: BMJ Publishing Group Ltd and British Society of Gastroenterology</publisher><subject>Antigens ; Carbohydrates ; Cell cycle ; Cell growth ; Cell Proliferation - drug effects ; Colorectal cancer ; defective denominators ; Diet ; Humans ; Intestinal Mucosa - cytology ; Intestinal Mucosa - microbiology ; Labeling ; large bowel ; Letter ; Microdissection - methods ; microflora ; Mitosis - physiology ; Mitotic Index ; Prebiotics ; Probiotics - pharmacology ; Proteins ; Senescence ; Studies</subject><ispartof>Gut, 2005-10, Vol.54 (10), p.1502-1502; author reply 1503</ispartof><rights>Copyright 2005 by Gut</rights><rights>Copyright: 2005 Copyright 2005 by Gut</rights><rights>Copyright © Copyright 2005 by Gut 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1774723/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1774723/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16162959$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Goodlad, R A</creatorcontrib><title>Defective denominators</title><title>Gut</title><addtitle>Gut</addtitle><description>[...]I would never recommend the use of proliferating cell nuclear antigen as a marker of cell proliferation as: (1) the method is difficult to standardise; (2) the antigen has a long half life; and (3) anomalous expression has been demonstrated in non-cycling near tumours and after administration of growth factors. 3 For sections, Ki67 is far better however even using this antibody the results of the present study are unlikely to be conclusive as only 2-4 crypts could be scored; for most studies I would recommend scoring 30 hemi crypts. 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subjects Antigens
Carbohydrates
Cell cycle
Cell growth
Cell Proliferation - drug effects
Colorectal cancer
defective denominators
Diet
Humans
Intestinal Mucosa - cytology
Intestinal Mucosa - microbiology
Labeling
large bowel
Letter
Microdissection - methods
microflora
Mitosis - physiology
Mitotic Index
Prebiotics
Probiotics - pharmacology
Proteins
Senescence
Studies
title Defective denominators
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