Analysis of the activation profile of dendritic cells derived from the bone marrow of interleukin‐12/interleukin‐23‐deficient mice

• Published in: Immunology 2005-04, Vol.114 (4), p.499-506
• Main Authors: Bastos, Karina R. B., De Deus Vieira de Moraes, Luciana, Zago, Cláudia A., Marinho, Cláudio R. F., Russo, Momtchilo, Alvarez, José M. M., D'Império Lima, Maria R.
• Format: Article
• Language: English
• Subjects: Animals; Bone Marrow - immunology; Cell Proliferation; Cells, Cultured; dendritic cells; Dendritic Cells - immunology; IL‐12; IL‐23; Immunophenotyping; Interferon-gamma - deficiency; Interferon-gamma - immunology; Interleukin-12 - deficiency; Interleukin-12 - immunology; Interleukin-23; Interleukin-23 Subunit p19; Interleukins - deficiency; Interleukins - immunology; Lymphocyte Activation - physiology; Mice; Mice, Inbred C57BL; Mice, Knockout; nitric oxide; Nitric Oxide - metabolism; Original; TGF‐β1; Transforming Growth Factor beta - metabolism
• ISSN: 0019-2805; 1365-2567
• DOI: 10.1111/j.1365-2567.2005.02118.x

Summary We have previously shown that macrophages from interleukin (IL)‐12p40 gene knockout (IL‐12/IL‐23–/–) mice have a bias towards the M2 activation profile, spontaneously secreting large quantities of transforming growth factor‐β1 (TGF‐β1) and producing low levels of nitric oxide (NO) in response to lipopolysaccharide (LPS) and interferon‐γ (IFN‐γ). To verify whether the activation profile of dendritic cells (DCs) is also influenced by the absence of IL‐12/IL‐23, bone marrow‐derived DCs from IL‐12/IL‐23–/– and C57BL/6 mice were evaluated. At first we noticed that ≈ 50% of the C57BL/6 DCs were dead after LPS‐induced maturation, whereas the mortality of IL‐12/IL‐23–/– DCs was