Home collection of urine specimens--boric acid bottles or Dipslides?

Sterile mid stream specimens of urine (MSSU) were obtained from 84 children in a hospital outpatient department. All 84 children collected urine at home by one of two Dipslide methods and by collection into boric acid within 24 hours of the hospital collected MSSU. The samples collected at home were...

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Published in:Archives of disease in childhood 1990-03, Vol.65 (3), p.286-289
Main Authors: Jewkes, F E, McMaster, D J, Napier, W A, Houston, I B, Postlethwaite, R J
Format: Article
Language:English
Subjects:
Acids
Adolescent
Analysis
Boric acid
Boric Acids
Child
Child, Preschool
Collections and collecting
Culture media
Diagnosis
Diagnostic specimens
Female
Home Nursing
Hospitals
Humans
Male
Medical examination
Pediatric diseases
Pediatrics
Specimen Handling - methods
Urinalysis
Urinary tract
Urinary tract infections
Urinary Tract Infections - diagnosis
Urinary tract infections in children
Urine
Urine - microbiology
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container_end_page 289
container_issue 3
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container_title Archives of disease in childhood
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creator Jewkes, F E
McMaster, D J
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Houston, I B
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description Sterile mid stream specimens of urine (MSSU) were obtained from 84 children in a hospital outpatient department. All 84 children collected urine at home by one of two Dipslide methods and by collection into boric acid within 24 hours of the hospital collected MSSU. The samples collected at home were posted to the hospital. Thirty six of the Dipslides (43%) and nine of the boric acid samples (10%) were not sterile but none had a pure growth of a single organism of greater than 10(5) organisms/ml. In addition, 17 of the Dipslides (20%) were returned with one or both media detached and therefore could not be relied upon to exclude urinary tract infection. In a second part to the study, 95 urines which showed a significant growth in primary culture were also cultured after storage in boric acid. Inhibition was noted in nine samples after storage in boric acid, seven of which were in underfilled bottles. Transport of specimens in boric acid produced less contamination than Dipslides but may inhibit growth in a small number of specimens. Technical failures with Dipslides were disappointingly high.
doi_str_mv 10.1136/adc.65.3.286
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All 84 children collected urine at home by one of two Dipslide methods and by collection into boric acid within 24 hours of the hospital collected MSSU. The samples collected at home were posted to the hospital. Thirty six of the Dipslides (43%) and nine of the boric acid samples (10%) were not sterile but none had a pure growth of a single organism of greater than 10(5) organisms/ml. In addition, 17 of the Dipslides (20%) were returned with one or both media detached and therefore could not be relied upon to exclude urinary tract infection. In a second part to the study, 95 urines which showed a significant growth in primary culture were also cultured after storage in boric acid. Inhibition was noted in nine samples after storage in boric acid, seven of which were in underfilled bottles. Transport of specimens in boric acid produced less contamination than Dipslides but may inhibit growth in a small number of specimens. 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All 84 children collected urine at home by one of two Dipslide methods and by collection into boric acid within 24 hours of the hospital collected MSSU. The samples collected at home were posted to the hospital. Thirty six of the Dipslides (43%) and nine of the boric acid samples (10%) were not sterile but none had a pure growth of a single organism of greater than 10(5) organisms/ml. In addition, 17 of the Dipslides (20%) were returned with one or both media detached and therefore could not be relied upon to exclude urinary tract infection. In a second part to the study, 95 urines which showed a significant growth in primary culture were also cultured after storage in boric acid. Inhibition was noted in nine samples after storage in boric acid, seven of which were in underfilled bottles. Transport of specimens in boric acid produced less contamination than Dipslides but may inhibit growth in a small number of specimens. 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subjects Acids
Adolescent
Analysis
Boric acid
Boric Acids
Child
Child, Preschool
Collections and collecting
Culture media
Diagnosis
Diagnostic specimens
Female
Home Nursing
Hospitals
Humans
Male
Medical examination
Pediatric diseases
Pediatrics
Specimen Handling - methods
Urinalysis
Urinary tract
Urinary tract infections
Urinary Tract Infections - diagnosis
Urinary tract infections in children
Urine
Urine - microbiology
title Home collection of urine specimens--boric acid bottles or Dipslides?
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