Searching for plasticity in dissociated cortical cultures on multi-electrode arrays

We attempted to induce functional plasticity in dense cultures of cortical cells using stimulation through extracellular electrodes embedded in the culture dish substrate (multi-electrode arrays, or MEAs). We looked for plasticity expressed in changes in spontaneous burst patterns, and in array-wide...

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Bibliographic Details
Published in:Journal of negative results in biomedicine 2006-10, Vol.5 (1), p.16-16, Article 16
Main Authors: Wagenaar, Daniel A, Pine, Jerome, Potter, Steve M
Format: Article
Language:English
Subjects:
Animals
Cells
Cells, Cultured
Cerebral Cortex - cytology
Electric Stimulation
Electrodes
Electrophysiology
Extracellular fluid
Microarray Analysis
Nervous system
Neuronal Plasticity - physiology
Neurons
Neurons - cytology
Neurons - physiology
Plasticity
Rats
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Summary:We attempted to induce functional plasticity in dense cultures of cortical cells using stimulation through extracellular electrodes embedded in the culture dish substrate (multi-electrode arrays, or MEAs). We looked for plasticity expressed in changes in spontaneous burst patterns, and in array-wide response patterns to electrical stimuli, following several induction protocols related to those used in the literature, as well as some novel ones. Experiments were performed with spontaneous culture-wide bursting suppressed by either distributed electrical stimulation or by elevated extracellular magnesium concentrations as well as with spontaneous bursting untreated. Changes concomitant with induction were no larger in magnitude than changes that occurred spontaneously, except in one novel protocol in which spontaneous bursts were quieted using elevated extracellular magnesium concentrations.
ISSN:1477-5751
1477-5751
DOI:10.1186/1477-5751-5-16