ClpS modulates but is not essential for bacterial N-end rule degradation

In eubacteria, the ClpS adaptor has been proposed to be essential for degradation of N-end rule substrates by the AAA(+) protease ClpAP. To test this model, we assayed degradation of substrates bearing N-end rule sequences isolated in a genetic screen for efficient degradation tags. ClpS was not vit...

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Bibliographic Details
Published in:Genes & development 2007-02, Vol.21 (4), p.403-408
Main Authors: Wang, Kevin H, Sauer, Robert T, Baker, Tania A
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Bacterial Proteins - metabolism
Carrier Proteins - genetics
Carrier Proteins - metabolism
Endopeptidase Clp - metabolism
Escherichia coli Proteins - genetics
Escherichia coli Proteins - metabolism
Eubacteria
Molecular Sequence Data
Research Communication
Substrate Specificity
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Summary:In eubacteria, the ClpS adaptor has been proposed to be essential for degradation of N-end rule substrates by the AAA(+) protease ClpAP. To test this model, we assayed degradation of substrates bearing N-end rule sequences isolated in a genetic screen for efficient degradation tags. ClpS was not vital for degradation in vivo but rather stimulated turnover in a sequence-specific manner. Although ClpS substantially enhanced degradation of N-end substrates at low substrate concentrations in vitro, it suppressed the degradation rate when substrate was saturating. Thus, we conclude that ClpAP recognizes N-end rule substrates directly, whereas ClpS modulates this degradation pathway.
ISSN:0890-9369
1549-5477
DOI:10.1101/gad.1511907