Measurement of soluble Fcγ receptor type IIIa derived from macrophages in plasma: increase in patients with rheumatoid arthritis

SUMMARY FcγRIII (CD16) is found in two alternative forms, a transmembrane FcγRIIIa expressed on NK cells and macrophages, and a glycosylphosphatidylinositol‐linked FcγRIIIb present on neutrophils. Previously, we measured soluble FcγRIIIa (sFcγRIIIa) in plasma of NA(1 +, 2‐) phenotyped donors with th...

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Published in:Clinical and experimental immunology 2003-06, Vol.132 (3), p.477-484
Main Authors: MASUDA, M., MORIMOTO, T., KOBATAKE, S., NISHIMURA, N., NAKAMOTO, K., DONG, X. H., KOMIYAMA, Y., OGAWA, R., TAKAHASHI, H.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Clinical Studies
Diseases of the osteoarticular system
Fc receptors
Fundamental and applied biological sciences. Psychology
human
Inflammation
Inflammatory joint diseases
macrophages
Medical sciences
Molecular and cellular biology
monocytes
rheumatoid arthritis
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Summary:SUMMARY FcγRIII (CD16) is found in two alternative forms, a transmembrane FcγRIIIa expressed on NK cells and macrophages, and a glycosylphosphatidylinositol‐linked FcγRIIIb present on neutrophils. Previously, we measured soluble FcγRIIIa (sFcγRIIIa) in plasma of NA(1 +, 2‐) phenotyped donors with the anti‐FcγRIII monoclonal antibody (MoAb) GRM1, which recognizes NA2‐FcγRIIIb and FcγRIIIa. The level of sFcγRIIIa, as well as the total sFcγRIII (sFcγRIIIa plus sFcγRIIIb) in patients with rheumatoid arthritis (RA) was significantly higher than that in healthy controls. In this study, we measured sFcγRIIIaMφ in plasma with a newly developed anti‐FcγRIII MoAb, MKGR14 (mIgM), which recognizes FcγRIIIaMφ specifically. From the recovery of purified sFcγRIIIaMφ, the amount of sFcγRIIIaMφ present was about half that of sFcγRIIIaNK, and that of sFcγRIIIa was about 50 times lower than that of sFcγRIIIb in pooled plasma from healthy NA(1 +, 2‐) phenotyped donors. The level of sFcγRIIIaMφ in RA patients was about four times higher than that in healthy controls. In RA patients, both the sFcγRIIIaMφ and sFcγRIIIa levels were increased as proportionally as the Lansbury Index. The sFcγRIIIa, but not sFcγRIIIaMφ levels, were increased directly proportional to C‐reactive protein. sFcγRIIIaMφ may be a novel marker of disease activity in RA.
ISSN:0009-9104
1365-2249
DOI:10.1046/j.1365-2249.2003.02168.x