CARD15 polymorphisms are associated with anti‐Saccharomyces cerevisiae antibodies in caucasian Crohn's disease patients

• Published in: Clinical and experimental immunology 2005-05, Vol.140 (2), p.354-359
• Main Authors: Cruyssen, B. Vander, Peeters, H., Hoffman, I. E. A., Laukens, D., Coucke, P., Marichal, D., Cuvelier, C., Remaut, E., Veys, E. M., Mielants, H., De Vos, M., De Keyser, F.
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; antibodies; Antibodies, Fungal - blood; ASCA; CARD15; caucasian; Clinical Studies; Crohn Disease - genetics; Crohn Disease - immunology; Crohn's disease; Enzyme-Linked Immunosorbent Assay - methods; Female; Genetic Markers; Genotype; Heterozygote; Humans; Intracellular Signaling Peptides and Proteins - genetics; Logistic Models; Male; Middle Aged; Nod2 Signaling Adaptor Protein; Polymorphism, Genetic; Saccharomyces cerevisiae - immunology
• ISSN: 0009-9104; 1365-2249
• DOI: 10.1111/j.1365-2249.2005.02759.x

Summary Carriage of CARD15 gene polymorphisms and the serological marker anti‐Saccharomyces cerevisiae antibodies (ASCA) are two markers for Crohn's disease (CD). Similar phenotypes have been associated with both markers. In the present study we analysed whether both markers were associated with each other and, if so, whether this association could be explained by a direct link or by an indirect association with those phenotypes. Therefore, we included 156 consecutive Caucasian CD patients and assessed the prevalence of the three common single nucleotide polymorphisms in the CARD15 gene. Serum samples were analysed for IgA and IgG ASCA by ELISA. CD patients with CARD15 polymorphisms were more frequently ASCA positive (OR 2·7 (1.4–5.2); P = 0·002) and had higher titres for ASCA IgA (P = 0·005) and ASCA IgG (P