Histological analysis of CD11c‐DTR/GFP mice after in vivo depletion of dendritic cells

Summary To investigate the dependence of individual immunological processes on DC, a transgenic mouse system (CD11c‐DTR/GFP mice) has been developed that allows conditional depletion of CD11c+ DC in vivo through administration of diphtheria toxin. We have performed careful histological analysis of C...

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Bibliographic Details
Published in:Clinical and experimental immunology 2005-09, Vol.141 (3), p.398-404
Main Authors: Probst, H. C., Tschannen, K., Odermatt, B., Schwendener, R., Zinkernagel, R. M., Van Den Broek, M.
Format: Article
Language:English
Subjects:
Animal Studies
Animals
Antimetabolites - administration & dosage
Biological and medical sciences
CD11c Antigen - immunology
CD11c‐DTR/GFP mice
Cell Count
Clodronic Acid - administration & dosage
conditional depletion
dendritic cells
Dendritic Cells - immunology
Diphtheria Toxin - administration & dosage
Female
Flow Cytometry
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Green Fluorescent Proteins - genetics
Heparin-binding EGF-like Growth Factor
histology
Immunohistochemistry - methods
Immunopathology
Intercellular Signaling Peptides and Proteins
Liposomes
Lymph Nodes - immunology
Lymphoid Tissue - immunology
macrophages
Macrophages - immunology
Male
Medical sciences
Mice
Mice, Inbred C57BL
Mice, Transgenic
Microscopy, Fluorescence
Models, Animal
Phagocytosis
Receptors, Cell Surface
Spleen - immunology
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Summary:Summary To investigate the dependence of individual immunological processes on DC, a transgenic mouse system (CD11c‐DTR/GFP mice) has been developed that allows conditional depletion of CD11c+ DC in vivo through administration of diphtheria toxin. We have performed careful histological analysis of CD11c‐DTR/GFP mice at different time points after diphtheria toxin injection and confirmed the transient depletion of CD11c+ cells from lymph nodes and spleen. Unexpectedly, the injection of diphtheria toxin completely depleted marginal zone and metallophilic MΦ from the spleen and their sinusoidal counterparts from the lymph nodes. This finding limits the use of CD11c‐DTR/GFP mice for the analysis of the role of DC to models and read outs that are proven to be independent of marginal zone and sinusoidal MΦ.
ISSN:0009-9104
1365-2249
DOI:10.1111/j.1365-2249.2005.02868.x