Influence of major surgery on the mannan‐binding lectin pathway of innate immunity

• Published in: Clinical and experimental immunology 2006-05, Vol.144 (2), p.239-246
• Main Authors: Ytting, H., Christensen, I. J., Basse, L., Lykke, J., Thiel, S., Jensenius, J. C., Nielsen, H. J.
• Format: Article
• Language: English
• Subjects: acute phase reactants; Acute-Phase Proteins - immunology; Acute-Phase Reaction - immunology; Aged; Aged, 80 and over; Biological and medical sciences; C-Reactive Protein - analysis; Clinical Studies; Cohort Studies; Colectomy - methods; Colonic Neoplasms - immunology; Colonic Neoplasms - surgery; Colorectal Neoplasms - immunology; Colorectal Neoplasms - surgery; complement; Complement Activation - immunology; Female; Fundamental and applied biological sciences. Psychology; Fundamental immunology; human; Humans; Immunity, Innate - immunology; Immunopathology; inflammation; Interleukin-6 - blood; Lectins - metabolism; Male; Mannans - metabolism; Mannose-Binding Protein-Associated Serine Proteases - analysis; Medical sciences; Middle Aged
• ISSN: 0009-9104; 1365-2249
• DOI: 10.1111/j.1365-2249.2006.03068.x

Summary The mannan‐binding lectin (MBL) pathway of complement activation is important in host defence against pathogens and possibly against cancer. We investigated the effect of major surgery on two central components of the MBL pathway; MBL and the MBL‐associated serine protease MASP‐2, and for comparison also measured the interleukin (IL)‐6 and C‐reactive protein (CRP) levels. Serial blood samples were obtained from patients belonging to two different cohorts. Cohort 1 comprised 60 patients undergoing open or laparoscopic colectomy for benign disease (n = 12) or colon cancer (n = 48). Cohort 2 comprised 27 patients undergoing elective, open surgery for colorectal cancer, and was included in order to cover blood sampling between days 2 and 6. As expected, the surgical stress induced a marked acute phase response, as evidenced by a large increase in IL‐6 (18‐fold) and CRP (13‐fold) levels with maximum at 12 h and 2 days, respectively. However, in both cohorts the levels of MBL and MBL‐associated serine protease 2 (MASP‐2) were largely unaffected, except for a minor but significant increase around day 8 in cohort 1. The preoperative levels of IL‐6 and CRP were correlated significantly in both cohorts (r = 0·71, P