Agreement of anti‐neutrophil cytoplasmic antibody measurements obtained from serum and plasma

Summary Serum and plasma are used interchangeably to measure anti‐neutrophil cytoplasmic antibodies (ANCA), even though the release of ANCA target antigens during the preparation of serum could affect ANCA assays and cause discrepancies between the results obtained from serum and plasma. To what ext...

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Bibliographic Details
Published in:Clinical and experimental immunology 2006-10, Vol.146 (1), p.15-20
Main Authors: Lee, A. S., Finkielman, J. D., Peikert, T., Hummel, A. M., Viss, M. A., Jacob, G. L., Homburger, H. A., Specks, U.
Format: Article
Language:English
Subjects:
antibodies
Antibodies, Antineutrophil Cytoplasmic - blood
anti‐neutrophil cytoplasmic
Biological and medical sciences
Biomarkers - blood
Blood Specimen Collection - methods
Clinical Studies
Enzyme-Linked Immunosorbent Assay - methods
enzyme‐linked immunosorbent assay
fluorescent antibody technique
Fluorescent Antibody Technique, Indirect - methods
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Granulomatosis with Polyangiitis - diagnosis
Humans
Immunopathology
Medical sciences
plasma
Plasma - immunology
Reproducibility of Results
serum
Serum - immunology
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Summary:Summary Serum and plasma are used interchangeably to measure anti‐neutrophil cytoplasmic antibodies (ANCA), even though the release of ANCA target antigens during the preparation of serum could affect ANCA assays and cause discrepancies between the results obtained from serum and plasma. To what extent ANCA test results obtained from serum agree and correlate with results from plasma remains unknown. Therefore, a comprehensive comparison was performed using serum and plasma samples which were collected in 175 patients with active Wegener's granulomatosis at enrolment of a recent randomized trial. These paired serum and plasma samples were subjected to parallel ANCA testing by standard indirect immunofluoresence on ethanol‐fixed neutrophils, a direct enzyme‐linked immunoassay (ELISA) for proteinase 3 (PR3)‐ANCA and myeloperoxidase (MPO)‐ANCA, and two different capture ELISAs for PR3‐ANCA. The concordance of categorical serum and plasma ANCA results was assessed using κ‐coefficients. These were > 0·8 for all assays, indicating a very good concordance between positive and negative serum and plasma results. Spearman's correlation coefficients for serum and plasma PR3‐ANCA values obtained by direct ELISA and both capture ELISAs were ≥ 0·95 (P 
ISSN:0009-9104
1365-2249
DOI:10.1111/j.1365-2249.2006.03179.x