The role of transcription in EGF- and FSH-mediated oocyte maturation in vitro

Understanding mechanisms responsible for meiotic resumption in mammalian oocytes is critical for the identification of strategies to enhance developmental competence of in vitro-matured oocytes. Improvement of in vitro oocyte maturation systems is dependent on a better understanding of mechanisms th...

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Bibliographic Details
Published in:Animal reproduction science 2007-03, Vol.98 (1), p.97-112
Main Authors: Farin, C.E., Rodriguez, K.F., Alexander, J.E., Hockney, J.E., Herrick, J.R., Kennedy-Stoskopf, S.
Format: Article
Language:English
Subjects:
animal reproduction
Animals
cell biology
Cell Culture Techniques
culture media
Dichlororibofuranosylbenzimidazole - pharmacology
EGF
embryo (animal)
embryo culture
embryogenesis
epidermal growth factor
Epidermal Growth Factor - pharmacology
Female
Follicle Stimulating Hormone - pharmacology
follicle-stimulating hormone
FSH
Gene transcription
Horses
in vitro fertilization
Mammals
Meiosis
Mice
Mitosis
Oocyte maturation
oocytes
Oocytes - drug effects
Oocytes - physiology
transcription (genetics)
Transcription, Genetic
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Summary:Understanding mechanisms responsible for meiotic resumption in mammalian oocytes is critical for the identification of strategies to enhance developmental competence of in vitro-matured oocytes. Improvement of in vitro oocyte maturation systems is dependent on a better understanding of mechanisms that regulate oocyte maturation both in vivo and in vitro as well as on the identification of methods to manipulate the meiotic progression of oocytes matured in vitro in a physiological manner. The purpose of this review is two-fold: first, to examine the mechanisms that underlie the acquisition of oocyte developmental competence and regulation of oocyte maturation in vivo and in vitro; second, to present data examining the role of transcription in mediating the ability of EGF and FSH to induce oocyte maturation in vitro. Results presented support the conclusions that (1) EGF-induced oocyte maturation does not require nascent gene transcription in both mice and domestic cats; (2) FSH requires gene transcription to induce oocyte maturation in both species; (3) EGF must be present in the maturation medium to optimize the effectiveness of FSH to promote oocyte maturation; (4) the mechanism used by FSH to induce oocyte maturation in vitro appears to predominate over that used by EGF when both EGF and FSH are present in maturation medium used for either murine or feline cumulus oocyte complexes.
ISSN:0378-4320
1873-2232
DOI:10.1016/j.anireprosci.2006.10.007