Co-occlusion and persistence of a baculovirus mutant lacking the polyhedrin gene

A co-occlusion process was evaluated as a commercially and ecologically acceptable strategy for the development of genetically improved baculovirus insecticides. Coinfection of Spodoptera frugiperda (IPLB-SF-21) tissue culture cells with Autographa californica nuclear polyhedrosis virus (AcMNPV) and...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 1990-10, Vol.56 (10), p.3057-3062
Main Authors: Hamblin, M. (Boyce Thompson Institute for Plant Research, Ithaca, NY), Van Beek, N.A.M, Hughes, P.R, Wood, H.A
Format: Article
Language:English
Subjects:
AUTOGRAPHA CALIFORNICA
AUXILIAIRE DE LUTTE BIOLOGIQUE
BACULOVIRIDAE
CULTIVO DE CELULAS
CULTURE DE CELLULE
GENE
General Microbial Ecology
GENES
insecticides
MUTANT
MUTANTES
ORGANISMOS PARA CONTROL BIOLOGICO
SPODOPTERA FRUGIPERDA
Trichoplusia ni
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Summary:A co-occlusion process was evaluated as a commercially and ecologically acceptable strategy for the development of genetically improved baculovirus insecticides. Coinfection of Spodoptera frugiperda (IPLB-SF-21) tissue culture cells with Autographa californica nuclear polyhedrosis virus (AcMNPV) and an AcMNPV mutant (Ac-E10) lacking the polyhedrin gene resulted in occlusion of both virus types within polyhedra. The amount of occluded Ac-E10 virions in progeny polyhedra populations during serial passage in Trichoplusia ni larvae was evaluated. Maintenance of the mutant in progeny polyhedra required polyhedra inocula containing equal numbers of the two virus types at a high dose. A significant reduction in occluded mutant nucleocapsids occurs with inoculum levels below a 100% lethal dose. At inoculum levels below a 30% lethal dose, the majority of fourth-instar larvae were infected with only one type of virus. The commercial application and ecological advantages of the co-occlusion process are discussed
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.56.10.3057-3062.1990