The effects of neosurugatoxin on evoked catecholamine secretion from bovine adrenal chromaffin cells

1 The effect of neosurugatoxin (NSTX), a toxin from the Japanese ivory mollusc (Babylonia japonica), on the nicotinic response of bovine adrenal chromaffin cells was examined. 2 NSTX inhibited acetylcholine‐and nicotine‐induced catecholamine secretion from the cultured cells with an IC50 against 5 μ...

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Bibliographic Details
Published in:British journal of pharmacology 1988-02, Vol.93 (2), p.275-280
Main Authors: Bourke, Jane E., Bunn, Stephen J., Marley, Philip D., Livett, Bruce G.
Format: Article
Language:English
Subjects:
Adrenal Glands - metabolism
Animal poisons toxicology. Antivenoms
Animals
Babylonia japonica
Biological and medical sciences
Catecholamines - metabolism
Cattle
Chromaffin System - cytology
Chromaffin System - metabolism
Epinephrine - metabolism
Ganglionic Blockers - pharmacology
Hexamethonium Compounds - pharmacology
In Vitro Techniques
Marine
Medical sciences
Mollusk Venoms - pharmacology
Nicotine - pharmacology
Norepinephrine - metabolism
Toxicology
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Summary:1 The effect of neosurugatoxin (NSTX), a toxin from the Japanese ivory mollusc (Babylonia japonica), on the nicotinic response of bovine adrenal chromaffin cells was examined. 2 NSTX inhibited acetylcholine‐and nicotine‐induced catecholamine secretion from the cultured cells with an IC50 against 5 μm nicotine of 30 nm. 3 This inhibitory effect was reversible and independent of the presence of agonist. 4 NSTX had no effect on the catecholamine release from cultured cells evoked by 50 mm K+, or 1 μm histamine. 5 NSTX had no effect on the stimulation of phosphatidylinositol metabolism evoked by 100 μm muscarine. 6 These results suggest NSTX may be useful as a nicotinic receptor probe in tissues such as the adrenal and sympathetic ganglia where α‐bungarotoxin is ineffective.
ISSN:0007-1188
1476-5381
DOI:10.1111/j.1476-5381.1988.tb11431.x