The contractile activities of lipoxin A4 and lipoxin B4 for guinea‐pig airway tissues

• Published in: British journal of pharmacology 1988-10, Vol.95 (2), p.562-568
• Main Authors: Jacques, Crawford A.J., Spur, Bernd W., Crea, Attilio E.G., Lee, Tak H.
• Format: Article
• Language: English
• Subjects: Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Chromones - pharmacology; Fundamental and applied biological sciences. Psychology; Guinea Pigs; Hydroxyeicosatetraenoic Acids - pharmacology; In Vitro Techniques; Leukotriene B4 - pharmacology; Lipids; Lipoxins; Lipoxygenase Inhibitors; Male; Muscle Contraction - drug effects; Muscle, Smooth - drug effects; Other biological molecules; SRS-A - antagonists & inhibitors; SRS-A - pharmacology; Stereoisomerism; Trachea - drug effects
• ISSN: 0007-1188; 1476-5381
• DOI: 10.1111/j.1476-5381.1988.tb11677.x

1 The isometric contractile activities of lipoxin A4 (LxA4) and lipoxin B4 (LxB4) were evaluated on guinea‐pig lung tissue over the concentration range, 10−8 to 10−5 m. 2 LxA4 contracted guinea‐pig lung parenchymal strips; the concentration eliciting 50% maximum histamine response was 3 × 10−6 m. LxA4 did not contract tracheal spirals. 3 The LxA4 dose‐response curve was parallel to that of leukotriene D4 (LTD4) with LxA4 being approximately 10,000 fold less potent than LTD4. 4 The time course of the contraction elicited by LxA4 was similar to that of LTD4 and it was slow in onset and did not plateau for 20 min. 5 Pre‐incubation of parenchymal strips with leukotriene receptor antagonists at a concentration of 1 × 10−6 m to 3 × 10−5 m FPL 55712 or 3 × 10−5 m L 649923 inhibited LxA4 activity. 6 Pre‐incubation of tissues with 1 × 10−5 m L 651392, a 5‐lipoxygenase inhibitor, or 1 × 10−5 m indomethacin, a cyclo‐oxygenase inhibitor, did not affect the contractile activity of LxA4. 7 LxB4 did not constrict parenchymal strips or tracheal spirals.