The effect of adrenalectomy on interleukin‐1 release in vitro and in vivo

• Published in: British journal of pharmacology 1989-12, Vol.98 (4), p.1137-1142
• Main Authors: Perretti, Mauro, Becherucci, Cristina, Scapigliati, Giuseppe, Parente, Luca
• Format: Article
• Language: English
• Subjects: 4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine - pharmacology; Adrenalectomy; Adrenals. Interrenals; Adrenomedullary hormones. Regulation; Animals; Biological and medical sciences; Cells, Cultured; Dexamethasone - pharmacology; Dinoprostone - metabolism; Freund's Adjuvant - pharmacology; Fundamental and applied biological sciences. Psychology; Humans; In Vitro Techniques; Indomethacin - pharmacology; Interleukin-1 - metabolism; Lipopolysaccharides - pharmacology; Macrophages - drug effects; Macrophages - metabolism; Precipitin Tests; Rats; Rats, Inbred Strains; Vertebrates: endocrinology
• ISSN: 0007-1188; 1476-5381
• DOI: 10.1111/j.1476-5381.1989.tb12657.x

1 Peritoneal macrophages (Mø) collected from adrenalectomized (ADX) rats released more interleukin‐1 (IL‐1) activity and prostaglandin E2 (PGE2) than macrophages from sham‐operated (SHO) rats. 2 The increase in IL‐1 activity in the supernatants was confirmed by the increase of the cell‐associated 33 kD IL‐1α precursor in ADX macrophages stimulated by lipopolysaccharide (LPS). 3 After the injection of Complete Freund's Adjuvant (CFA) to induce adjuvant arthritis, 60% of the ADX rats died, while no deaths occurred in the SHO group. 4 The in vivo administration of dexamethasone inhibited both IL‐1 and PGE2 release by macrophages as well as protecting ADX animals from CFA‐induced death. Indomethacin and BW 755C partially protected the animals from this lethal effect. 5 These results suggest that adrenalectomy induces an increased release of IL‐1 both in vitro and in vivo, and are consistent with a feedback mechanism between IL‐1 and glucocorticoid hormones.