nup154 Genetically interacts with cup and plays a cell-type-specific function during Drosophila melanogaster egg-chamber development

Nucleoporin Nup154 is a Drosophila component of the nuclear pore complex (NPC), evolutionarily conserved from yeast to humans. While functional studies carried out in both yeast and metazoan cells indicated that Nup154 homologs are key elements of the NPC framework, the striking phenotypic specifici...

Full description

Saved in:
Bibliographic Details
Published in:Genetics (Austin) 2007-04, Vol.175 (4), p.1751-1759
Main Authors: Grimaldi, M.R, Cozzolino, L, Malva, C, Graziani, F, Gigliotti, S
Format: Article
Language:English
Subjects:
alleles
Animals
Animals, Genetically Modified
Chromosomes
Drosophila melanogaster
Drosophila melanogaster - cytology
Drosophila melanogaster - genetics
Drosophila melanogaster - growth & development
Drosophila melanogaster - metabolism
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
egg chamber
Female
Gene Expression Regulation, Developmental
Genes, Insect
Genotype & phenotype
Green Fluorescent Proteins - genetics
insect development
Investigations
Metazoa
mutants
Nuclear Pore Complex Proteins - genetics
Nuclear Pore Complex Proteins - metabolism
nucleoporins
nurse cells
oogenesis
Oogenesis - genetics
Oogenesis - physiology
ovaries
phenotype
protein-protein interactions
Proteins
Recombinant Proteins - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Nucleoporin Nup154 is a Drosophila component of the nuclear pore complex (NPC), evolutionarily conserved from yeast to humans. While functional studies carried out in both yeast and metazoan cells indicated that Nup154 homologs are key elements of the NPC framework, the striking phenotypic specificity displayed by nup154 hypomorphic mutant alleles suggested that Nup154 might play additional roles in the context of the NPC. Actually, genetic analyses demonstrated that mutant nurse-cell nuclei do not undergo a normal chromosome dispersal process, uncovering an essential requirement for nup154 gene function during oogenesis. In this report, we show that Nup154 interacts genetically and physically with Cup, a germline-specific protein implicated in multiple aspects of female gametogenesis, including the regulation of the nurse-cell chromosome structure. The two proteins colocalize in vivo and are co-immunoprecipitated from ovarian extracts. Moreover, cup, nup154 double mutants exhibit much stronger oogenesis defects than single mutants. Our findings delineate an intriguing scenario where an ubiquitous nucleoporin might directly influence specialized developmental events.
ISSN:0016-6731
1943-2631
1943-2631
DOI:10.1534/genetics.106.062844