Measurement of inflammatory cytokines by multicytokine assay in tears of patients with glaucoma topically treated with chronic drugs

• Published in: British journal of ophthalmology 2007-01, Vol.91 (1), p.29-32
• Main Authors: Malvitte, L, Montange, T, Vejux, A, Baudouin, C, Bron, A M, Creuzot-Garcher, C, Lizard, G
• Format: Article
• Language: English
• Subjects: Administration, Topical; Aged; Amides - administration & dosage; Antihypertensive Agents - administration & dosage; BAC; benzalkonium chloride; Bimatoprost; Biological and medical sciences; Brimonidine Tartrate; Carteolol - administration & dosage; Case-Control Studies; Chemokines - analysis; Clinical Science - Scientific Report; Cloprostenol - administration & dosage; Cloprostenol - analogs & derivatives; Cytokines; Cytokines - analysis; Dose-Response Relationship, Immunologic; Drug Therapy, Combination; Drugs; Female; Glaucoma; Glaucoma - drug therapy; Glaucoma - immunology; Glaucoma and intraocular pressure; Granulocytes; HLA; human leucocyte antigen; Humans; IFN; interferon; Intraocular Pressure - drug effects; Laboratories; Life Sciences; Lipids - administration & dosage; macrophage inflammatory protein; Male; MCP; Medical sciences; MIP; Miscellaneous; monocyte chemotactic protein; Ophthalmology; Prospective Studies; Prostaglandins F, Synthetic - administration & dosage; Quinoxalines - administration & dosage; Software; Sulfonamides - administration & dosage; Tears - immunology; Thiophenes - administration & dosage; Timolol - administration & dosage; Tumor necrosis factor-TNF
• ISSN: 0007-1161; 1468-2079
• DOI: 10.1136/bjo.2006.101485

Aim: To investigate the ocular surface inflammatory response to chronic topical treatments in patients with glaucoma by measuring the cytokine level in tears using multiplex bead analysis. Methods: Tear samples were collected from 21 patients with glaucoma and 12 healthy volunteers. Tears were analysed for the presence of 17 cytokines: interleukin (IL)1β, IL2, IL4, IL5, IL6, IL7, IL8, IL10, IL12, IL13, IL17, granulocyte-colony stimulating factor, granulocyte-macrophage stimulating factor, interferon (INF)γ, monocyte chemotactic protein (MCP)1, macrophage inflammatory protein 1β and tumour necrosis factor (TNF)α. The cytokines in each sample of tears were measured using multiplex bead analysis with microspheres as solid support for immunoassays. Results: In the tears of treated patients, proinflammatory cytokines (IL1β, IL6, IL12, TNFα) were significantly increased compared with controls. T helper (Th)1 (INFγ, IL2) and Th2 (IL5, IL10, IL4) type cytokines were also significantly higher (p