Genetic Polymorphisms Influence Plasmodium ovale PCR Detection Accuracy

Detection of Plasmodium ovale by use of a nested PCR assay with a novel Plasmodium ovale primer set was superior to detection of Plasmodium ovale by real-time PCR assays. Nested PCR was also better at detecting P. malariae. The detection of P. ovale in many patients first admitted >2 months follo...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Clinical Microbiology 2007-05, Vol.45 (5), p.1624-1627
Main Authors: Calderaro, A, Piccolo, G, Perandin, F, Gorrini, C, Peruzzi, S, Zuelli, C, Ricci, L, Manca, N, Dettori, G, Chezzi, C, Snounou, G
Format: Article
Language:English
Subjects:
Adult
Animals
Biological and medical sciences
Child
Child, Preschool
Fundamental and applied biological sciences. Psychology
Humans
Infectious diseases
Malaria - diagnosis
Malaria - parasitology
Medical sciences
Microbiology
Middle Aged
Parasitology
Plasmodium ovale
Plasmodium ovale - genetics
Plasmodium ovale - isolation & purification
Polymerase Chain Reaction - methods
Polymorphism, Genetic
Reproducibility of Results
RNA, Protozoan - genetics
RNA, Ribosomal - genetics
Sensitivity and Specificity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Detection of Plasmodium ovale by use of a nested PCR assay with a novel Plasmodium ovale primer set was superior to detection of Plasmodium ovale by real-time PCR assays. Nested PCR was also better at detecting P. malariae. The detection of P. ovale in many patients first admitted >2 months following their return to Italy indicated that P. ovale relapses are common.
ISSN:0095-1137
1098-660X
1098-5530
DOI:10.1128/JCM.02316-06