Single-molecule mass spectrometry in solution using a solitary nanopore

We introduce a two-dimensional method for mass spectrometry in solution that is based on the interaction between a nanometer-scale pore and analytes. As an example, poly(ethylene glycol) molecules that enter a single α-hemolysin pore cause distinct mass-dependent conductance states with characterist...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2007-05, Vol.104 (20), p.8207-8211
Main Authors: Robertson, Joseph W.F, Rodrigues, Claudio G, Stanford, Vincent M, Rubinson, Kenneth A, Krasilnikov, Oleg V, Kasianowicz, John J
Format: Article
Language:English
Subjects:
Applied statistics
Bacterial Toxins - chemistry
Biological Sciences
Biophysics
Capillaries
Electric Conductivity
Electric current
Glycols
Hemolysin Proteins - chemistry
Histograms
Mass spectra
Mass spectrometry
Mass spectroscopy
Membranes
Molecular Weight
Molecules
Nanostructures - chemistry
Physical Sciences
Polymers
Proteins
Solutions - chemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Time Factors
Time series
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Summary:We introduce a two-dimensional method for mass spectrometry in solution that is based on the interaction between a nanometer-scale pore and analytes. As an example, poly(ethylene glycol) molecules that enter a single α-hemolysin pore cause distinct mass-dependent conductance states with characteristic mean residence times. The conductance-based mass spectrum clearly resolves the repeat unit of ethylene glycol, and the mean residence time increases monotonically with the poly(ethylene glycol) mass. This technique could prove useful for the real-time characterization of molecules in solution.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.0611085104