Isolation of Leptospira noguchii from sheep

The main goal of this study was to obtain new isolates of Leptospira spp. from sheep. A total of 10 kidney samples and 44 blood samples were collected from sheep slaughtered in Pelotas, Southern Brazil. One isolate was obtained which was identified by 16S rRNA gene sequencing and serogrouping to be...

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Bibliographic Details
Published in:Veterinary microbiology 2007-03, Vol.121 (1), p.144-149
Main Authors: Silva, Éverton F., Brod, Claudiomar S., Cerqueira, Gustavo M., Bourscheidt, Débora, Seyffert, Núbia, Queiroz, Adriano, Santos, Cleiton S., Ko, Albert I., Dellagostin, Odir A.
Format: Article
Language:English
Subjects:
Agglutination Tests - veterinary
Animals
Antibodies, Bacterial - blood
Bacteriology
Base Sequence
Biological and medical sciences
Brazil
Cricetinae
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
epidemiological studies
Fundamental and applied biological sciences. Psychology
Isolation
Kidney Diseases - blood
Kidney Diseases - epidemiology
Kidney Diseases - microbiology
Kidney Diseases - veterinary
Leptospira
Leptospira - genetics
Leptospira - isolation & purification
Leptospira noguchii
Leptospirosis
Leptospirosis - epidemiology
Leptospirosis - microbiology
Leptospirosis - veterinary
Microbiology
Miscellaneous
molecular epidemiology
Molecular Sequence Data
nucleotide sequences
pathogen identification
Phylogeny
Polymerase Chain Reaction - veterinary
Prevalence
RNA, Ribosomal, 16S - chemistry
RNA, Ribosomal, 16S - genetics
Sequence Alignment
sequence analysis
Serogrouping
Sheep
sheep diseases
Sheep Diseases - epidemiology
Sheep Diseases - microbiology
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Summary:The main goal of this study was to obtain new isolates of Leptospira spp. from sheep. A total of 10 kidney samples and 44 blood samples were collected from sheep slaughtered in Pelotas, Southern Brazil. One isolate was obtained which was identified by 16S rRNA gene sequencing and serogrouping to be Leptospira noguchii serogroup Autumnalis. Microscopic agglutination test (MAT) evaluation revealed that 4.5% of the sheep sera reacted against the Autumnalis serogroup. This is the first report of isolation of L. noguchii from sheep. Together these findings indicate that L. noguchii infections may be a potentially important veterinary problem in this domestic animal species.
ISSN:0378-1135
1873-2542
DOI:10.1016/j.vetmic.2006.11.010