Imbalance of the interleukin 1 system in colonic mucosa—association with intestinal inflammation and interleukin 1 receptor agonist genotype 2

• Published in: Gut 1997-11, Vol.41 (5), p.651-657
• Main Authors: Andus, T, Daig, R, Vogl, D, Aschenbrenner, E, Lock, G, Hollerbach, S, Köllinger, M, Schölmerich, J, Gross, V
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Biopsy; Colon; Crohn’s disease; Cytokines; Gastroenterology. Liver. Pancreas. Abdomen; genotype; Genotype & phenotype; Immune system; Inflammation; Inflammatory; Inflammatory bowel disease; interleukin 1; interleukin 1 receptor antagonist; Medical sciences; mucosal inflammation; Other diseases. Semiology; Proteins; Rodents; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus; Studies; Ulcers
• ISSN: 0017-5749; 1468-3288; 1458-3288
• DOI: 10.1136/gut.41.5.651

Background—Interleukin 1 (IL-1) α and β are potent cytokines which play key roles in inflammation. They are controlled by IL-1 receptor antagonist (IL-1ra). Aims—To investigate the influence of mucosal inflammation and IL-1ra genotype on the IL-1ra:IL-1 balance. Patients and methods—IL-1α, IL-1β, and IL-1ra were measured by enzyme linked immunosorbent assay (ELISA) in biopsy specimens taken from inflamed and non-inflamed colon of 60 patients with Crohn’s disease (CD), 34 with ulcerative colitis (UC), 15 inflammatory controls, and 103 non-inflamed controls. IL-1ra genotype was determined by polymerase chain reaction and gel electrophoresis. Results—IL-1α and IL-1β were significantly increased in inflamed mucosa in inflammatory bowel disease (IBD) (CD: 53.5 (22.4) and 409.9 (118.7) pg/mg protein, respectively; UC: 18.9 (6.8) and 214.5 (78.2) pg/mg, respectively) and non-IBD patients (19.2 (7.4) and 281.4 (121.0) pg/mg, respectively; p