Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals

The resazurin assay utilising microtitre-plate, described by Drummond and Waigh in 2000, has been modified to achieve more accuracy in the determination of the minimum inhibitory concentration (MIC) values of natural products, including crude extracts, chromatographic fractions or purified compounds...

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Bibliographic Details
Published in:Methods (San Diego, Calif.) Calif.), 2007-08, Vol.42 (4), p.321-324
Main Authors: Sarker, Satyajit D., Nahar, Lutfun, Kumarasamy, Yashodharan
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - chemistry
Anti-Bacterial Agents - pharmacology
Antibacterial assay
Bacteria - drug effects
Cell Proliferation
Cells, Cultured
Drug Evaluation, Preclinical - methods
Indicators and Reagents - chemistry
Microbial Sensitivity Tests - methods
Microchemistry
Molecular Structure
Oxazines - chemistry
Oxazines - pharmacology
Plant extract
Plants, Medicinal - chemistry
Reproducibility of Results
Resazurin
Serial dilution
Xanthenes - chemistry
Xanthenes - pharmacology
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Summary:The resazurin assay utilising microtitre-plate, described by Drummond and Waigh in 2000, has been modified to achieve more accuracy in the determination of the minimum inhibitory concentration (MIC) values of natural products, including crude extracts, chromatographic fractions or purified compounds against various bacterial strains. This modified resazurin method is simple, sensitive, rapid, robust and reliable, and could be used successfully to assess antibacterial properties of natural products.
ISSN:1046-2023
1095-9130
DOI:10.1016/j.ymeth.2007.01.006