Label-Free Detection of Mitochondrial Distribution in Cells by Nonresonant Raman Microspectroscopy

High spatial resolution Raman maps of fixed cells in an aqueous environment are reported. These maps were obtained by collecting individual Raman spectra via a Raman microspectrometer in a raster pattern on a 0.5-μm grid and assembling pseudocolor maps from the spectral hypercubes by multivariate me...

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Bibliographic Details
Published in:Biophysical journal 2007-07, Vol.93 (2), p.668-673
Main Authors: Matthäus, Christian, Chernenko, Tatyana, Newmark, Judith A., Warner, Carol M., Diem, Max
Format: Article
Language:English
Subjects:
Aldehydes
Aqueous environments
Assembling
Biophysical Phenomena
Biophysics
Cells
Cellular biology
Correlation
Fluorescence
Fluorescent Dyes
HeLa Cells
Histology
Humans
Hypercubes
Microscopy, Fluorescence
Mitochondria
Mitochondria - chemistry
Mitochondria - physiology
Mitochondria - ultrastructure
Mitochondrial DNA
Spectra
Spectroscopy, Imaging, Other Techniques
Spectrum analysis
Spectrum Analysis, Raman
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Summary:High spatial resolution Raman maps of fixed cells in an aqueous environment are reported. These maps were obtained by collecting individual Raman spectra via a Raman microspectrometer in a raster pattern on a 0.5-μm grid and assembling pseudocolor maps from the spectral hypercubes by multivariate methods. The Raman maps show the nucleus and the nucleoli of cells as well as subcellular organization in the cytoplasm. In particular, the distribution of mitochondria in the perinuclear region could be demonstrated by correlating distinct areas of the Raman maps with corresponding areas of fluorescence maps of the same cells after staining with mitochondria-specific labels. To the best of our knowledge, this is the first report of label-free detection of mitochondria inside a somatic mammalian cell using Raman microspectroscopy.
ISSN:0006-3495
1542-0086
DOI:10.1529/biophysj.106.102061