The Human Hematopoietic Stem Cell Compartment Is Heterogeneous for CXCR4 Expression

The chemokine stromal derived factor-1α (SDF-1α) has been implicated recently in the chemotaxis of primitive human hematopoietic cells, suggesting that pluripotent human stem cells express the SDF-1α receptor, CXCR4. By using flow cytometry and confocal microscopy, we have identified and isolated pr...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2000-12, Vol.97 (26), p.14626-14631
Main Authors: Rosu-Myles, M, Gallacher, L, Murdoch, B, Hess, D A, Keeney, M, Kelvin, D, Dale, L, Ferguson, S S, Wu, D, Fellows, F, Bhatia, M
Format: Article
Language:English
Subjects:
Animals
Biological Sciences
Blood cells
CD34 antigen
Cell Compartmentation
Cells
Chemokine CXCL12
Chemokines, CXC - metabolism
CXCR4 protein
Hematopoietic stem cells
Hematopoietic Stem Cells - classification
Hematopoietic Stem Cells - metabolism
Hematopoietic Stem Cells - physiology
Humans
Medical research
Mice
Mice, Inbred NOD
Mice, SCID
Muscle, Skeletal - cytology
Myeloid cells
Neurons - cytology
Pluripotent stem cells
Receptors, CXCR4 - biosynthesis
Stem cell transplantation
Stem cells
stromal cell-derived factor 1^a
Transplantation
Xenotransplantation
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Summary:The chemokine stromal derived factor-1α (SDF-1α) has been implicated recently in the chemotaxis of primitive human hematopoietic cells, suggesting that pluripotent human stem cells express the SDF-1α receptor, CXCR4. By using flow cytometry and confocal microscopy, we have identified and isolated primitive subsets of human CXCR4+and CXCR4-cells. Distinctions in the progenitor content and response to SDF-1α in vitro indicate that CXCR4+and CXCR4-cells represent discrete populations of primitive blood cells. The i.v. transplantation of these subfractions into immune-deficient mice established that both possess comparable engraftment capacity in vivo. Human myeloid, lymphoid, and primitive CD34+CXCR4+cells were present in chimeric animals transplanted with either subset, indicating that CXCR4+and CXCR4-stem cells have equivalent proliferative and differentiative abilities. Our study indicates that the human stem cell compartment is heterogeneous for CXCR4 expression, suggesting that the relationship between CXCR4 expression and stem cell repopulating function is not obligatory.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.97.26.14626