Plasticity-Induced Growth of Dendritic Spines by Exocytic Trafficking from Recycling Endosomes

Dendritic spines are micron-sized membrane protrusions receiving most excitatory synaptic inputs in the mammalian brain. Spines form and grow during long-term potentiation (LTP) of synaptic strength. However, the source of membrane for spine formation and enlargement is unknown. Here we report that...

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Bibliographic Details
Published in:Neuron (Cambridge, Mass.) Mass.), 2006-12, Vol.52 (5), p.817-830
Main Authors: Park, Mikyoung, Salgado, Jennifer M., Ostroff, Linnaea, Helton, Thomas D., Robinson, Camenzind G., Harris, Kristen M., Ehlers, Michael D.
Format: Article
Language:English
Subjects:
Animals
Biological Transport, Active - physiology
Cell culture
Cell Size
CELLBIO
Dendritic Spines - physiology
Dendritic Spines - ultrastructure
DNA - biosynthesis
DNA - genetics
Electrophysiology
Endosomes - physiology
Endosomes - ultrastructure
Exocytosis - physiology
Hippocampus - cytology
Hippocampus - growth & development
Image Processing, Computer-Assisted
Immunohistochemistry
Kinases
Long-Term Potentiation - physiology
Microscopy
Microscopy, Electron
Microscopy, Electron, Transmission
MOLNEURO
Morphogenesis
Neuronal Plasticity - physiology
Neurons
Plasma
Proteins
Rats
Receptors, N-Methyl-D-Aspartate - physiology
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Summary:Dendritic spines are micron-sized membrane protrusions receiving most excitatory synaptic inputs in the mammalian brain. Spines form and grow during long-term potentiation (LTP) of synaptic strength. However, the source of membrane for spine formation and enlargement is unknown. Here we report that membrane trafficking from recycling endosomes is required for the growth and maintenance of spines. Using live-cell imaging and serial section electron microscopy, we demonstrate that LTP-inducing stimuli promote the mobilization of recycling endosomes and vesicles into spines. Preventing recycling endosomal transport abolishes LTP-induced spine formation. Using a pH-sensitive recycling cargo, we show that exocytosis from recycling endosomes occurs locally in spines, is triggered by activation of synaptic NMDA receptors, and occurs concurrently with spine enlargement. Thus, recycling endosomes provide membrane for activity-dependent spine growth and remodeling, defining a novel membrane trafficking mechanism for spine morphological plasticity and providing a mechanistic link between structural and functional plasticity during LTP.
ISSN:0896-6273
1097-4199
DOI:10.1016/j.neuron.2006.09.040