Viral load and CD69 molecule expression on freshly isolated and cultured mitogen‐stimulated lymphocytes of children with perinatal HIV‐1 infection

HIV‐1 in adults changes the proportion of mitogen‐stimulated lymphocytes expressing the CD69 activation molecule, but little is known about this molecule expression on lymphocytes of HIV‐1‐infected (HIV‐1+) children. Freshly isolated CD3+, CD4+, CD8+ and CD19+ and phytohaemagglutinin (PHA)‐stimulate...

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Published in:Clinical and experimental immunology 1999-09, Vol.117 (3), p.513-516
Main Authors: DE MARTINO, M, ROSSI, M. E, AZZARI, C, GELLI, M. G, CHIARELLI, F, GALLI, L, VIERUCCI, A
Format: Article
Language:English
Subjects:
activation molecules
AIDS/HIV
Antigens, CD - analysis
Antigens, CD - biosynthesis
Antigens, Differentiation, T-Lymphocyte - biosynthesis
Biological and medical sciences
CD69
Child
Child, Preschool
Cross-Sectional Studies
Female
Follow-Up Studies
HIV Infections - immunology
HIV Infections - transmission
HIV Infections - virology
HIV-1 - genetics
HIV-1 - immunology
HIV‐1
HIV‐1 load
Human immunodeficiency virus 1
Human viral diseases
Humans
Infant
Infectious Disease Transmission, Vertical
Infectious diseases
Lectins, C-Type
Lymphocytes - drug effects
Lymphocytes - immunology
Lymphocytes - virology
Male
Medical sciences
Mitogens - pharmacology
Original
Perinatal Care
perinatal HIV‐1 infection
Phytohemagglutinins - pharmacology
Prospective Studies
Viral diseases
Viral diseases of the lymphoid tissue and the blood. Aids
Viral Load
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Summary:HIV‐1 in adults changes the proportion of mitogen‐stimulated lymphocytes expressing the CD69 activation molecule, but little is known about this molecule expression on lymphocytes of HIV‐1‐infected (HIV‐1+) children. Freshly isolated CD3+, CD4+, CD8+ and CD19+ and phytohaemagglutinin (PHA)‐stimulated CD3+, CD4+ and CD8+ lymphocytes co‐expressing CD69 were investigated cross‐sectionally (adopting a MoAb double‐staining technique) in 24 HIV‐1+ children with severe disease and given anti‐retroviral therapy and in 24 age‐matched healthy children. CD69 results in HIV‐1+ children were correlated with plasma HIV‐1 RNA load prospectively determined. HIV‐1+ compared with healthy children had higher frequencies of freshly isolated CD3+CD69+ (2.4 ± 2.2% versus 0.9 ± 0.5%; P = 0.002) and CD8+CD69+ (1.5 ± 1.1% versus 0.5 ± 0.2%; P 
ISSN:0009-9104
1365-2249
DOI:10.1046/j.1365-2249.1999.01011.x