DNA vaccination favours memory rather than effector B cell responses

Following priming and boosting of mice with a DNA vector pEE6ΔS‐hCGß expressing sequences encoding a transmembrane version of the β‐chain of human chorionic gonadotropin (hCGβ), we failed to detect appreciable levels of specific antibody. However, subsequent challenge with hCG protein in Ribi adjuva...

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Bibliographic Details
Published in:Clinical and experimental immunology 1999-07, Vol.117 (1), p.106-112
Main Authors: LAYLOR, R, PORAKISHVILI, N, DE SOUZA, J. B, PLAYFAIR, J. H. L, DELVES, P. J, LUND, T
Format: Article
Language:English
Subjects:
Adjuvants, Immunologic
Animals
B-Lymphocyte Subsets - immunology
Biological and medical sciences
Chorionic Gonadotropin, beta Subunit, Human - genetics
Chorionic Gonadotropin, beta Subunit, Human - immunology
DNA immunization
Female
Genetic Vectors - genetics
Genetic Vectors - immunology
hCG
Humans
Immunization
Immunization, Secondary
Immunologic Memory
immunological memory
Immunopathology
Immunotherapy (general aspects)
Interferon-gamma - metabolism
Interleukin-4 - metabolism
Medical sciences
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Original
Spleen - immunology
Th1 Cells - metabolism
Th2 Cells - metabolism
vaccine
Vaccines, DNA - immunology
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Summary:Following priming and boosting of mice with a DNA vector pEE6ΔS‐hCGß expressing sequences encoding a transmembrane version of the β‐chain of human chorionic gonadotropin (hCGβ), we failed to detect appreciable levels of specific antibody. However, subsequent challenge with hCG protein in Ribi adjuvant elicited a strong and rapid secondary immune response. This response was of comparable magnitude to that produced following priming, boosting and challenge with protein in adjuvant. Thus, DNA vaccination with this vector is as efficient in generating B cell memory as is conventional immunization, but the memory generation occurs in the absence of an overt effector response. Despite an overall similar level of specific antibody, the DNA‐vaccinated mice produced hCG‐specific antibodies biased towards IgG2a and IgG2b isotypes, whereas the protein‐vaccinated mice produced higher levels of IgG1 antibodies. Both Th1 and Th2 cytokines (interferon‐gamma (IFN‐γ) and IL‐4) were lower in the spleens of the DNA‐immunized animals compared with the protein‐Ribi‐immunized animals, possibly suggesting a different level of helper T cell response to the two different modes of immunization.
ISSN:0009-9104
1365-2249
DOI:10.1046/j.1365-2249.1999.00941.x