The 19‐kD antigen and protective immunity in a murine model of tuberculosis

The 19‐kD antigen is a cell wall‐associated lipoprotein present in Mycobacterium tuberculosis and in bacille Calmette–Guérin (BCG) vaccine strains. Expression of the 19‐kD antigen as a recombinant protein in two saprophytic mycobacteria—M. vaccae and M. smegmatis—resulted in abrogation of their abil...

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Bibliographic Details
Published in:Clinical and experimental immunology 2000-05, Vol.120 (2), p.274-279
Main Authors: Yeremeev, V. V., Lyadova, I. V., Nikonenko, B. V., Apt, A. S., Abou‐Zeid, C., Inwald, J., Young, D. B.
Format: Article
Language:English
Subjects:
Animals
Antigens, Bacterial - genetics
Antigens, Bacterial - immunology
Bacterial Proteins - genetics
Bacterial Proteins - immunology
BCG Vaccine - genetics
BCG Vaccine - immunology
Disease Models, Animal
immune response
Immunity to Infection
lipoproteins
Mice
Mice, Inbred C57BL
mouse model
Mycobacterium - immunology
Mycobacterium smegmatis
Mycobacterium smegmatis - immunology
Mycobacterium tuberculosis
Mycobacterium tuberculosis - immunology
Mycobacterium vaccae
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - immunology
recombinant vaccines
Time Factors
tuberculosis
Tuberculosis - microbiology
Tuberculosis - physiopathology
Tuberculosis - prevention & control
Vaccination
Vaccines, DNA - genetics
Vaccines, DNA - immunology
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Summary:The 19‐kD antigen is a cell wall‐associated lipoprotein present in Mycobacterium tuberculosis and in bacille Calmette–Guérin (BCG) vaccine strains. Expression of the 19‐kD antigen as a recombinant protein in two saprophytic mycobacteria—M. vaccae and M. smegmatis—resulted in abrogation of their ability to confer protection against M. tuberculosis in a murine challenge model, and in their ability to prime a DTH response to cross‐reactive mycobacterial antigens. Induction of an immune response to the 19‐kD antigen by an alternative approach of DNA vaccination had no effect on subsequent M. tuberculosis challenge. These results are consistent with a model in which the presence of the 19‐kD protein has a detrimental effect on the efficacy of vaccination with live mycobacteria. Targeted inactivation of genes encoding selected antigens represents a potential route towards development of improved vaccine candidates.
ISSN:0009-9104
1365-2249
DOI:10.1046/j.1365-2249.2000.01212.x