Characterization of T cell clones derived from lymph nodes and lungs of Pseudomonas aeruginosa‐susceptible and resistant mice following immunization with heat‐killed bacteria

• Published in: Clinical and experimental immunology 2000-08, Vol.121 (2), p.275-282
• Main Authors: Kondratieva, T. K., Kobets, N. V., Khaidukov, S. V., Yeremeev, V. V., Lyadova, I. V., Apt, A. S., Tam, M. F., Stevenson, M. M.
• Format: Article
• Language: English
• Subjects: AIDS/HIV; Animals; Antigens, Bacterial - immunology; Bacterial Vaccines - immunology; CD4 antigen; Cells, Cultured; Clone Cells - immunology; cytokines; g-Interferon; Genetic Predisposition to Disease; Histocompatibility Antigens Class II - immunology; Immunity to Infection; Immunity, Innate; Immunophenotyping; Interferon-gamma - metabolism; Interleukin-10 - metabolism; Interleukin-4 - metabolism; Lung - pathology; lungs; Lymph Nodes - pathology; Lymphocyte Activation; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Pseudomonas aeruginosa; Pseudomonas aeruginosa - immunology; Pseudomonas Infections - prevention & control; specificity; T cell clones; T-Lymphocyte Subsets - immunology; T-Lymphocyte Subsets - metabolism; Th1 Cells - immunology; Th1 Cells - metabolism; Th2 Cells - immunology; Th2 Cells - metabolism; Vaccination; Vaccines, Inactivated - immunology
• ISSN: 0009-9104; 1365-2249
• DOI: 10.1046/j.1365-2249.2000.01222.x

Pseudomonas aeruginosa‐resistant BALB/c and susceptible C57Bl/6 (B6) mice were immunized with heat‐killed Pseudomonas either in the foot pad or via the trachea, and panels of Pseudomonas‐specific T cell clones were developed from lymph nodes and lungs. All clones from either strain, whether of lymph node or lung origin, were CD3+CD4+CD8−TCRαβ+. The efficacy of cloning from lymph node cells was comparable between BALB/c and B6 mice. All lymph node BALB/c clones proliferated in response to Pseudomonas antigen in a dose‐dependent manner, and this response was MHC class II‐restricted. Vigorous proliferation by a considerable proportion of B6 T cell clones occurred in the absence of specific antigen. Lymph node clones from either strain could be categorized as either Th1 or Th0 on the basis of interferon‐gamma (IFN‐γ)/IL‐4 production. In either mouse strain the efficacy of cloning from lung tissue was substantially lower than from lymph nodes, but the efficacy of cloning from BALB/c compared with B6 lungs was higher. Four lung T cell clones from BALB/c and two from B6 mice were expanded for further analyses, and an interstrain difference was observed in cytokine production. Both B6 lung T cell clones were Th1‐like and produced IFN‐γ but not IL‐4 and IL‐10, whereas four BALB/c lung T cell clones were Th2‐like and produced IL‐4 and IL‐10 but not IFN‐γ. These observations suggest that differences in the CD4+ Th response in the lung may contribute to differences among inbred mouse strains in the level of resistance to bronchopulmonary Pseudomonas infection.