Effect of murine recombinant interleukin‐5 on the cell population in guinea‐pig airways

• Published in: British journal of pharmacology 1992-01, Vol.105 (1), p.19-22
• Main Authors: Iwama, T., Nagai, H., Suda, H., Tsuruoka, N., Koda, A.
• Format: Article
• Language: English
• Subjects: airway cell populations; airway inflammation; Animals; anti‐asthmatic drugs; Asthma - drug therapy; Asthma - pathology; Biological and medical sciences; Bronchoalveolar Lavage Fluid - cytology; Cell Count - drug effects; Epithelial Cells; Epithelium - drug effects; Guinea Pigs; Interleukin-5 - pharmacology; Interleukin‐5 (IL‐5); Leukocyte Count - drug effects; Medical sciences; Mice; Pharmacology. Drug treatments; Recombinant Proteins - pharmacology; Respiratory system; Trachea - cytology; Trachea - drug effects
• ISSN: 0007-1188; 1476-5381
• DOI: 10.1111/j.1476-5381.1992.tb14204.x

1 An intratracheal injection of murine recombinant interleukin 5 (mrIL‐5, 2–15 μg/0.25 ml/animal) induced a dose‐dependent increase in the number of macrophages, eosinophils, neutrophils and epithelial cells in the bronchoalveolar lavage fluid (BALF) of guinea‐pigs 24 h after administration. Bovine serum albumin (15 μg/0.25 ml/animal), used as a reference material, did not cause any change of this type. 2 The intratracheal administration of mrIL‐5 at a dose of 15 μg showed a tendency to increase the number of these pulmonary inflammatory cells and epithelial cells in the BALF at 12 h with a significant increase observed at 24 h. 3 Prednisolone (20 mg kg−1, i.p.) inhibited the mrIL‐5‐induced increase in macrophages, eosinophils, neutrophils and epithelial cells. Ketotifen (2 mg kg−1, i.p.) reduced the mrIL‐5‐induced increase in the eosinophil, neutrophil and epithelial cell populations. The simultaneous injection of 2% disodium cromoglycate (DSCG) into the trachea prevented the mrIL‐5‐induced increase in the number of airway epithelial cells, without affecting changes in the other inflammatory leukocytes. 4 These results suggest that mrIL‐5 is a potent inducer of lung inflammation, in terms of increased inflammatory leukocytes and epithelial cells in guinea‐pig BALF. Prednisolone, DSCG and ketotifen are effective against mrIL‐5‐induced pulmonary inflammation, especially the desquamation of bronchial epithelial cells.