The effect of a tachykinin NK1 receptor antagonist, SR140333, on oedema formation induced in rat skin by venom from the Phoneutria nigriventer spider

• Published in: British journal of pharmacology 1996-05, Vol.118 (2), p.295-298
• Main Authors: Palframan, R.T., Costa, S.K.P., Wilsoncroft, P., Antunes, E., Nucci, G., Brain, S.D.
• Format: Article
• Language: English
• Subjects: Animal poisons toxicology. Antivenoms; Animals; Biological and medical sciences; Edema - chemically induced; Edema - prevention & control; Male; Medical sciences; Methysergide - pharmacology; Neurokinin-1 Receptor Antagonists; NK1 receptors; oedema; Peptide Fragments - pharmacology; Phoneutria nigriventer venom; Piperidines - pharmacology; Pyrilamine - pharmacology; Quinuclidines - pharmacology; Rats; Rats, Wistar; Receptors, Neurokinin-1 - agonists; skin; Skin Diseases - chemically induced; Skin Diseases - prevention & control; Spider Venoms - toxicity; SR140333; Substance P - analogs & derivatives; Substance P - pharmacology; tachykinins; Toxicology
• ISSN: 0007-1188; 1476-5381
• DOI: 10.1111/j.1476-5381.1996.tb15402.x

1 The possibility that tachykinin NK1 receptors are involved in the plasma extravasation evoked by intradermal (i.d.) injection of Phoneutria nigriventer venom (PNV) in rat dorsal skin in vivo has been investigated. 2 Local oedema formation induced by the i.d. injection of test agents was measured by the extravascular accumulation of intravenously (i.v.) injected 125I‐labelled human serum albumin over a 30 min period. 3 The tachykinin NK1 agonist, GR73632 (30 pmol per site), induced local oedema formation which was potentiated by co‐injection with the neuropeptide vasodilator, calcitonin gene‐related peptide (CGRP, 10 pmol per site). The non‐peptide tachykinin NK1 receptor antagonist, SR140333 (0.03‐1 nmol per site co‐injected, i.d.) significantly inhibited (0.3 nmol per site, P