Two Stereoisomers of Spheroidene in the Rhodobacter sphaeroides R26 Reaction Center: A DFT Analysis of Resonance Raman Spectra

From a theoretical analysis of the resonance Raman spectra of 19 isotopomers of spheroidene reconstituted into the reaction center (RC) of Rhodobacter sphaeroides R26, we conclude that the carotenoid in the RC occurs in two configurations. The normal mode underlying the resonance Raman transition at...

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Bibliographic Details
Published in:Biophysical journal 2007-08, Vol.93 (3), p.981-991
Main Authors: Wirtz, A.C., van Hemert, M.C., Lugtenburg, J., Frank, H.A., Groenen, E.J.J.
Format: Article
Language:English
Subjects:
Biochemistry
Biophysics
Carbon
Carotenoids - analysis
Carotenoids - chemistry
Hydrogen
Photosynthesis
Photosynthetic Reaction Center Complex Proteins - analysis
Photosynthetic Reaction Center Complex Proteins - chemistry
Reproducibility of Results
Rhodobacter sphaeroides
Rhodobacter sphaeroides - metabolism
Spectroscopy, Imaging, Other Techniques
Spectrum Analysis, Raman - methods
Stereoisomerism
X-Ray Diffraction
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Summary:From a theoretical analysis of the resonance Raman spectra of 19 isotopomers of spheroidene reconstituted into the reaction center (RC) of Rhodobacter sphaeroides R26, we conclude that the carotenoid in the RC occurs in two configurations. The normal mode underlying the resonance Raman transition at 1239 cm −1, characteristic for spheroidene in the RC, has been identified and found to uniquely refer to the cis nature of the 15,15′ carbon-carbon double bond. Detailed analysis of the isotope-induced shifts of transitions in the 1500–1550 cm −1 region proves that, besides the 15,15′- cis configuration, spheroidene in the RC adopts another cis-configuration, most likely the 13,14- cis configuration.
ISSN:0006-3495
1542-0086
DOI:10.1529/biophysj.106.103473